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Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China

BACKGROUND: Anaplasmosis is caused by obligate intracellular bacteria in the genus Anaplasma. These bacterial pathogens are transmitted by ticks and impact both human and animal health. This study was conducted to determine the prevalence and molecular characterization of Anaplasma spp. in ruminants...

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Autores principales: Yang, Jifei, Li, Youquan, Liu, Zhijie, Liu, Junlong, Niu, Qingli, Ren, Qiaoyun, Chen, Ze, Guan, Guiquan, Luo, Jianxun, Yin, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344993/
https://www.ncbi.nlm.nih.gov/pubmed/25889906
http://dx.doi.org/10.1186/s13071-015-0727-3
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author Yang, Jifei
Li, Youquan
Liu, Zhijie
Liu, Junlong
Niu, Qingli
Ren, Qiaoyun
Chen, Ze
Guan, Guiquan
Luo, Jianxun
Yin, Hong
author_facet Yang, Jifei
Li, Youquan
Liu, Zhijie
Liu, Junlong
Niu, Qingli
Ren, Qiaoyun
Chen, Ze
Guan, Guiquan
Luo, Jianxun
Yin, Hong
author_sort Yang, Jifei
collection PubMed
description BACKGROUND: Anaplasmosis is caused by obligate intracellular bacteria in the genus Anaplasma. These bacterial pathogens are transmitted by ticks and impact both human and animal health. This study was conducted to determine the prevalence and molecular characterization of Anaplasma spp. in ruminants sampled in Xinjiang, northwest China. METHODS: A survey was performed in August 2012 in rural areas of six counties in Xinjiang province. A total of 250 blood samples from ruminants were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. RESULTS: The results showed a high prevalence of Anaplasma spp. in ruminants, with at least three different Anaplasma species detected (A. phagocytophilum, A. bovis and A. ovis). The mean prevalence of single infection with each species was 17.6% (A. phagocytophilum), 4.8% (A. bovis) and 40.5% (A. ovis). Coinfection occurred in 20 (8.0%) animals. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. phagocytophilum revealed a higher degree of genetic diversity for the latter. The results for A. ovis showed genotypic variation among geographic regions in China. In addition, a closely related isolate to the canine pathogen A. platys was identified in ruminants. CONCLUSIONS: This survey revealed a high prevalence of Anaplasma sp. infections in sheep and cattle in the northwestern border regions of China, indicating the potential risk of transboundary disease.
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spelling pubmed-43449932015-03-02 Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China Yang, Jifei Li, Youquan Liu, Zhijie Liu, Junlong Niu, Qingli Ren, Qiaoyun Chen, Ze Guan, Guiquan Luo, Jianxun Yin, Hong Parasit Vectors Research BACKGROUND: Anaplasmosis is caused by obligate intracellular bacteria in the genus Anaplasma. These bacterial pathogens are transmitted by ticks and impact both human and animal health. This study was conducted to determine the prevalence and molecular characterization of Anaplasma spp. in ruminants sampled in Xinjiang, northwest China. METHODS: A survey was performed in August 2012 in rural areas of six counties in Xinjiang province. A total of 250 blood samples from ruminants were collected and tested for the presence of Anaplasma spp. by PCR. Positive samples were genetically characterized based on the 16S rRNA and msp4 genes. RESULTS: The results showed a high prevalence of Anaplasma spp. in ruminants, with at least three different Anaplasma species detected (A. phagocytophilum, A. bovis and A. ovis). The mean prevalence of single infection with each species was 17.6% (A. phagocytophilum), 4.8% (A. bovis) and 40.5% (A. ovis). Coinfection occurred in 20 (8.0%) animals. Phylogenetic analysis of the 16S rRNA gene of A. bovis and A. phagocytophilum revealed a higher degree of genetic diversity for the latter. The results for A. ovis showed genotypic variation among geographic regions in China. In addition, a closely related isolate to the canine pathogen A. platys was identified in ruminants. CONCLUSIONS: This survey revealed a high prevalence of Anaplasma sp. infections in sheep and cattle in the northwestern border regions of China, indicating the potential risk of transboundary disease. BioMed Central 2015-02-19 /pmc/articles/PMC4344993/ /pubmed/25889906 http://dx.doi.org/10.1186/s13071-015-0727-3 Text en © Yang et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Yang, Jifei
Li, Youquan
Liu, Zhijie
Liu, Junlong
Niu, Qingli
Ren, Qiaoyun
Chen, Ze
Guan, Guiquan
Luo, Jianxun
Yin, Hong
Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China
title Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China
title_full Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China
title_fullStr Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China
title_full_unstemmed Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China
title_short Molecular detection and characterization of Anaplasma spp. in sheep and cattle from Xinjiang, northwest China
title_sort molecular detection and characterization of anaplasma spp. in sheep and cattle from xinjiang, northwest china
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344993/
https://www.ncbi.nlm.nih.gov/pubmed/25889906
http://dx.doi.org/10.1186/s13071-015-0727-3
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