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Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium

BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors su...

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Autores principales: Moghadam, Farshad Homayouni, Tayebi, Tahereh, Dehghan, Maryam, Eslami, Gilda, Nadri, Hamid, Moradi, Alireza, Vahedian-Ardakani, Hassanali, Barzegar, Kazem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345293/
https://www.ncbi.nlm.nih.gov/pubmed/25774263
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author Moghadam, Farshad Homayouni
Tayebi, Tahereh
Dehghan, Maryam
Eslami, Gilda
Nadri, Hamid
Moradi, Alireza
Vahedian-Ardakani, Hassanali
Barzegar, Kazem
author_facet Moghadam, Farshad Homayouni
Tayebi, Tahereh
Dehghan, Maryam
Eslami, Gilda
Nadri, Hamid
Moradi, Alireza
Vahedian-Ardakani, Hassanali
Barzegar, Kazem
author_sort Moghadam, Farshad Homayouni
collection PubMed
description BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated METHODS: MSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs & P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels. RESULTS: Staining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups. CONCLUSION: This study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage.
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spelling pubmed-43452932015-03-13 Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium Moghadam, Farshad Homayouni Tayebi, Tahereh Dehghan, Maryam Eslami, Gilda Nadri, Hamid Moradi, Alireza Vahedian-Ardakani, Hassanali Barzegar, Kazem Int J Hematol Oncol Stem Cell Res Original Article BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) are one of the undifferentiated multipotential cell sources of human body. MSCs have the capacity to form a variety of cell types, especially chondrocytes and osteocytes. Learning about responses of MSCs to external milieu and chemical factors such as pH could recommend new approaches for preparation of suitable scaffolds for bone and cartilage tissue engineering. In present study, the effect of alkaline medium on chondrogenic and osteogenic differentiation of rat MSCs was evaluated METHODS: MSCs were harvested from bone marrow of animals and then the response of passage1 and 2 of MSCs (P1 MSCs & P2 MSCs) to the culture in alkaline medium (pH: 8) was evaluated. Cytochemical and immunocytochemical staining were performed to distinguish chondrocytes and osteocytes. Real-time PCR was performed to evaluate the type II collagen and osteopontin mRNA levels. RESULTS: Staining for type II collagen, a chondrocytic specific marker, revealed that after one-week culture in alkaline medium, a considerable amount of P1 MSCs had shown chondrocytic morphology. By prolonging the culture period up to 4 weeks, osteogenic cells with expanded matrix and mineralized areas around them were appeared. Results of real-time PCR showed that P1 MSCs after one week culture in alkaline medium expressed highest rate of type II collagen and osteopontin mRNA among all groups. CONCLUSION: This study demonstrated that alkaline medium is a potent chondrogenic differentiation inducer for MSCs in their first passage. Tehran University of Medical Sciences 2014-10-01 /pmc/articles/PMC4345293/ /pubmed/25774263 Text en Copyright: © International Journal of Hematology-Oncology and Stem Cell Research & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Moghadam, Farshad Homayouni
Tayebi, Tahereh
Dehghan, Maryam
Eslami, Gilda
Nadri, Hamid
Moradi, Alireza
Vahedian-Ardakani, Hassanali
Barzegar, Kazem
Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
title Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
title_full Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
title_fullStr Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
title_full_unstemmed Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
title_short Differentiation of Bone Marrow Mesenchymal Stem Cells into Chondrocytes after Short Term Culture in Alkaline Medium
title_sort differentiation of bone marrow mesenchymal stem cells into chondrocytes after short term culture in alkaline medium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345293/
https://www.ncbi.nlm.nih.gov/pubmed/25774263
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