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Aptamer Selection Technology and Recent Advances
Over the last decade, aptamers have begun to find their way from basic research to diverse commercial applications. The development of diagnostics is even more widespread than clinical applications because aptamers do not have to be extensively modified to enhance their in vivo stability and pharmac...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345306/ https://www.ncbi.nlm.nih.gov/pubmed/28110747 http://dx.doi.org/10.1038/mtna.2014.74 |
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author | Blind, Michael Blank, Michael |
author_facet | Blind, Michael Blank, Michael |
author_sort | Blind, Michael |
collection | PubMed |
description | Over the last decade, aptamers have begun to find their way from basic research to diverse commercial applications. The development of diagnostics is even more widespread than clinical applications because aptamers do not have to be extensively modified to enhance their in vivo stability and pharmacokinetics in diagnostic assays. The increasing attention has propelled the technical progress of the in vitro selection technology (SELEX) to enhance the efficiency of developing aptamers for commercially interesting targets. This review highlights recent progress in the technical steps of a SELEX experiment with a focus on high-throughput next-generation sequencing and bioinformatics. Achievements have been made in the optimization of aptamer libraries, separation schemes, amplification of the selected libraries and the identification of aptamer sequences from enriched libraries. |
format | Online Article Text |
id | pubmed-4345306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-43453062015-03-09 Aptamer Selection Technology and Recent Advances Blind, Michael Blank, Michael Mol Ther Nucleic Acids Review Over the last decade, aptamers have begun to find their way from basic research to diverse commercial applications. The development of diagnostics is even more widespread than clinical applications because aptamers do not have to be extensively modified to enhance their in vivo stability and pharmacokinetics in diagnostic assays. The increasing attention has propelled the technical progress of the in vitro selection technology (SELEX) to enhance the efficiency of developing aptamers for commercially interesting targets. This review highlights recent progress in the technical steps of a SELEX experiment with a focus on high-throughput next-generation sequencing and bioinformatics. Achievements have been made in the optimization of aptamer libraries, separation schemes, amplification of the selected libraries and the identification of aptamer sequences from enriched libraries. Nature Publishing Group 2015-01 2015-01-13 /pmc/articles/PMC4345306/ /pubmed/28110747 http://dx.doi.org/10.1038/mtna.2014.74 Text en Copyright © 2015 American Society of Gene & Cell Therapy http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Review Blind, Michael Blank, Michael Aptamer Selection Technology and Recent Advances |
title | Aptamer Selection Technology and Recent Advances |
title_full | Aptamer Selection Technology and Recent Advances |
title_fullStr | Aptamer Selection Technology and Recent Advances |
title_full_unstemmed | Aptamer Selection Technology and Recent Advances |
title_short | Aptamer Selection Technology and Recent Advances |
title_sort | aptamer selection technology and recent advances |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345306/ https://www.ncbi.nlm.nih.gov/pubmed/28110747 http://dx.doi.org/10.1038/mtna.2014.74 |
work_keys_str_mv | AT blindmichael aptamerselectiontechnologyandrecentadvances AT blankmichael aptamerselectiontechnologyandrecentadvances |