Cargando…

Aptamer Selection Technology and Recent Advances

Over the last decade, aptamers have begun to find their way from basic research to diverse commercial applications. The development of diagnostics is even more widespread than clinical applications because aptamers do not have to be extensively modified to enhance their in vivo stability and pharmac...

Descripción completa

Detalles Bibliográficos
Autores principales: Blind, Michael, Blank, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345306/
https://www.ncbi.nlm.nih.gov/pubmed/28110747
http://dx.doi.org/10.1038/mtna.2014.74
_version_ 1782359565241679872
author Blind, Michael
Blank, Michael
author_facet Blind, Michael
Blank, Michael
author_sort Blind, Michael
collection PubMed
description Over the last decade, aptamers have begun to find their way from basic research to diverse commercial applications. The development of diagnostics is even more widespread than clinical applications because aptamers do not have to be extensively modified to enhance their in vivo stability and pharmacokinetics in diagnostic assays. The increasing attention has propelled the technical progress of the in vitro selection technology (SELEX) to enhance the efficiency of developing aptamers for commercially interesting targets. This review highlights recent progress in the technical steps of a SELEX experiment with a focus on high-throughput next-generation sequencing and bioinformatics. Achievements have been made in the optimization of aptamer libraries, separation schemes, amplification of the selected libraries and the identification of aptamer sequences from enriched libraries.
format Online
Article
Text
id pubmed-4345306
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-43453062015-03-09 Aptamer Selection Technology and Recent Advances Blind, Michael Blank, Michael Mol Ther Nucleic Acids Review Over the last decade, aptamers have begun to find their way from basic research to diverse commercial applications. The development of diagnostics is even more widespread than clinical applications because aptamers do not have to be extensively modified to enhance their in vivo stability and pharmacokinetics in diagnostic assays. The increasing attention has propelled the technical progress of the in vitro selection technology (SELEX) to enhance the efficiency of developing aptamers for commercially interesting targets. This review highlights recent progress in the technical steps of a SELEX experiment with a focus on high-throughput next-generation sequencing and bioinformatics. Achievements have been made in the optimization of aptamer libraries, separation schemes, amplification of the selected libraries and the identification of aptamer sequences from enriched libraries. Nature Publishing Group 2015-01 2015-01-13 /pmc/articles/PMC4345306/ /pubmed/28110747 http://dx.doi.org/10.1038/mtna.2014.74 Text en Copyright © 2015 American Society of Gene & Cell Therapy http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
spellingShingle Review
Blind, Michael
Blank, Michael
Aptamer Selection Technology and Recent Advances
title Aptamer Selection Technology and Recent Advances
title_full Aptamer Selection Technology and Recent Advances
title_fullStr Aptamer Selection Technology and Recent Advances
title_full_unstemmed Aptamer Selection Technology and Recent Advances
title_short Aptamer Selection Technology and Recent Advances
title_sort aptamer selection technology and recent advances
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345306/
https://www.ncbi.nlm.nih.gov/pubmed/28110747
http://dx.doi.org/10.1038/mtna.2014.74
work_keys_str_mv AT blindmichael aptamerselectiontechnologyandrecentadvances
AT blankmichael aptamerselectiontechnologyandrecentadvances