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PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos
During Xenopus early development, FGF signaling is involved in mesoderm formation and neurogenesis by modulating various signaling cascades. FGF-MAPK signaling induces Xbra expression, which maintains mesodermal fate through an autocatalytic-loop. Interestingly, previous reports have demonstrated th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society for Biochemistry and Molecular Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345511/ https://www.ncbi.nlm.nih.gov/pubmed/24499677 http://dx.doi.org/10.5483/BMBRep.2014.47.12.290 |
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author | Yoon, Jaeho Kim, Jung-Ho Lee, Sung-Young Kim, SungChan Park, Jae-Bong Lee, Jae-Yong Kim, Jaebong |
author_facet | Yoon, Jaeho Kim, Jung-Ho Lee, Sung-Young Kim, SungChan Park, Jae-Bong Lee, Jae-Yong Kim, Jaebong |
author_sort | Yoon, Jaeho |
collection | PubMed |
description | During Xenopus early development, FGF signaling is involved in mesoderm formation and neurogenesis by modulating various signaling cascades. FGF-MAPK signaling induces Xbra expression, which maintains mesodermal fate through an autocatalytic-loop. Interestingly, previous reports have demonstrated that basic FGF (bFGF) treatment alone does not induce neurogenesis in ectodermal explants, even though FGF signaling inhibits BMP signaling via phosphorylation in Smad1 linker region. In addition, the overexpression of dominantnegative Xbra induces neurogenesis in ectodermal explants. However, the detailed mechanism underlying these phenomena has not yet been clarified. In this work, we showed that bFGF-Xbra signaling increased the PV.1 expression. DN-Xbra was found to decrease PV.1 expression, and the co-injection of PV.1 with DN-Xbra reduced neurogenesis in ectodermal explants. Furthermore, the knockdown of PV.1 induced neurogenesis in bFGF-treated ectodermal explants. Taken together, our results demonstrate that FGF-Xbra signaling induces PV.1 expression and that PV.1 functions as a neural repressor in the FGF-treated ectoderm. [BMB Reports 2014; 47(12): 673-678] |
format | Online Article Text |
id | pubmed-4345511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Korean Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-43455112015-03-02 PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos Yoon, Jaeho Kim, Jung-Ho Lee, Sung-Young Kim, SungChan Park, Jae-Bong Lee, Jae-Yong Kim, Jaebong BMB Rep Research-Articles During Xenopus early development, FGF signaling is involved in mesoderm formation and neurogenesis by modulating various signaling cascades. FGF-MAPK signaling induces Xbra expression, which maintains mesodermal fate through an autocatalytic-loop. Interestingly, previous reports have demonstrated that basic FGF (bFGF) treatment alone does not induce neurogenesis in ectodermal explants, even though FGF signaling inhibits BMP signaling via phosphorylation in Smad1 linker region. In addition, the overexpression of dominantnegative Xbra induces neurogenesis in ectodermal explants. However, the detailed mechanism underlying these phenomena has not yet been clarified. In this work, we showed that bFGF-Xbra signaling increased the PV.1 expression. DN-Xbra was found to decrease PV.1 expression, and the co-injection of PV.1 with DN-Xbra reduced neurogenesis in ectodermal explants. Furthermore, the knockdown of PV.1 induced neurogenesis in bFGF-treated ectodermal explants. Taken together, our results demonstrate that FGF-Xbra signaling induces PV.1 expression and that PV.1 functions as a neural repressor in the FGF-treated ectoderm. [BMB Reports 2014; 47(12): 673-678] Korean Society for Biochemistry and Molecular Biology 2014-12 /pmc/articles/PMC4345511/ /pubmed/24499677 http://dx.doi.org/10.5483/BMBRep.2014.47.12.290 Text en Copyright © 2014, Korean Society for Biochemistry and Molecular Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research-Articles Yoon, Jaeho Kim, Jung-Ho Lee, Sung-Young Kim, SungChan Park, Jae-Bong Lee, Jae-Yong Kim, Jaebong PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos |
title | PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos |
title_full | PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos |
title_fullStr | PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos |
title_full_unstemmed | PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos |
title_short | PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos |
title_sort | pv.1 induced by fgf-xbra functions as a repressor of neurogenesis in xenopus embryos |
topic | Research-Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345511/ https://www.ncbi.nlm.nih.gov/pubmed/24499677 http://dx.doi.org/10.5483/BMBRep.2014.47.12.290 |
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