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RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT
RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation, adhesion, and migration of cells. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. On the other hand, other...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nagoya University
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345693/ https://www.ncbi.nlm.nih.gov/pubmed/25741038 |
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author | KISHIMOTO, MAYUKO MATSUDA, TAKENORI YANASE, SHOUGO KATSUMI, AKIRA SUZUKI, NOBUAKI IKEJIRI, MAKOTO TAKAGI, AKIRA IKAWA, MASAHITO KOJIMA, TETSUHITO KUNISHIMA, SHINJI KIYOI, HITOSHI NAOE, TOMOKI MATSUSHITA, TADASHI MARUYAMA, MITSUO |
author_facet | KISHIMOTO, MAYUKO MATSUDA, TAKENORI YANASE, SHOUGO KATSUMI, AKIRA SUZUKI, NOBUAKI IKEJIRI, MAKOTO TAKAGI, AKIRA IKAWA, MASAHITO KOJIMA, TETSUHITO KUNISHIMA, SHINJI KIYOI, HITOSHI NAOE, TOMOKI MATSUSHITA, TADASHI MARUYAMA, MITSUO |
author_sort | KISHIMOTO, MAYUKO |
collection | PubMed |
description | RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation, adhesion, and migration of cells. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. On the other hand, other members of the Rho GTPase family, such as Cdc42, RhoA, and Rac, have been intensively studied and are known to be required for B cell development in the bone marrow and spleen. We hypothesized that RhoF is also involved in B cell development. To examine our hypothesis, we analyzed B cell development in RhoF knockout (KO) mice and found a significant reduction in marginal zone (MZ) B cells in the spleen, although T cell development in the thymus and spleen was not affected. Consistent with these results, the width of the MZ B cell region in the spleen was significantly reduced in the RhoF KO mice. However, the antigen-specific antibody titer of IgM and IgG3 after MZ B cell-specific antigen (T cell-independent antigen, type I) stimulation was not affected by RhoF deletion. Furthermore, we demonstrated that RhoF was dispensable for stromal cell-derived factor-1α- and B lymphocyte chemoattractant-induced B cell migration. These results suggest that RhoF promotes MZ B cell development in the spleen. |
format | Online Article Text |
id | pubmed-4345693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nagoya University |
record_format | MEDLINE/PubMed |
spelling | pubmed-43456932015-03-04 RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT KISHIMOTO, MAYUKO MATSUDA, TAKENORI YANASE, SHOUGO KATSUMI, AKIRA SUZUKI, NOBUAKI IKEJIRI, MAKOTO TAKAGI, AKIRA IKAWA, MASAHITO KOJIMA, TETSUHITO KUNISHIMA, SHINJI KIYOI, HITOSHI NAOE, TOMOKI MATSUSHITA, TADASHI MARUYAMA, MITSUO Nagoya J Med Sci Original Paper RhoF is a member of the Rho GTPase family that has been implicated in various cell functions including long filopodia formation, adhesion, and migration of cells. Although RhoF is expressed in lymphoid tissues, the roles of RhoF in B cell development remain largely unclear. On the other hand, other members of the Rho GTPase family, such as Cdc42, RhoA, and Rac, have been intensively studied and are known to be required for B cell development in the bone marrow and spleen. We hypothesized that RhoF is also involved in B cell development. To examine our hypothesis, we analyzed B cell development in RhoF knockout (KO) mice and found a significant reduction in marginal zone (MZ) B cells in the spleen, although T cell development in the thymus and spleen was not affected. Consistent with these results, the width of the MZ B cell region in the spleen was significantly reduced in the RhoF KO mice. However, the antigen-specific antibody titer of IgM and IgG3 after MZ B cell-specific antigen (T cell-independent antigen, type I) stimulation was not affected by RhoF deletion. Furthermore, we demonstrated that RhoF was dispensable for stromal cell-derived factor-1α- and B lymphocyte chemoattractant-induced B cell migration. These results suggest that RhoF promotes MZ B cell development in the spleen. Nagoya University 2014-08 /pmc/articles/PMC4345693/ /pubmed/25741038 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view the details of this license, please visit (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Paper KISHIMOTO, MAYUKO MATSUDA, TAKENORI YANASE, SHOUGO KATSUMI, AKIRA SUZUKI, NOBUAKI IKEJIRI, MAKOTO TAKAGI, AKIRA IKAWA, MASAHITO KOJIMA, TETSUHITO KUNISHIMA, SHINJI KIYOI, HITOSHI NAOE, TOMOKI MATSUSHITA, TADASHI MARUYAMA, MITSUO RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT |
title | RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT |
title_full | RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT |
title_fullStr | RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT |
title_full_unstemmed | RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT |
title_short | RHOF PROMOTES MURINE MARGINAL ZONE B CELL DEVELOPMENT |
title_sort | rhof promotes murine marginal zone b cell development |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345693/ https://www.ncbi.nlm.nih.gov/pubmed/25741038 |
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