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Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
Neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. In order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346111/ https://www.ncbi.nlm.nih.gov/pubmed/25884945 http://dx.doi.org/10.1186/s13567-014-0139-y |
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author | Arranz-Solís, David Benavides, Julio Regidor-Cerrillo, Javier Fuertes, Miguel Ferre, Ignacio Ferreras, Maria del Carmen Collantes-Fernández, Esther Hemphill, Andrew Pérez, Valentín Ortega-Mora, Luis Miguel |
author_facet | Arranz-Solís, David Benavides, Julio Regidor-Cerrillo, Javier Fuertes, Miguel Ferre, Ignacio Ferreras, Maria del Carmen Collantes-Fernández, Esther Hemphill, Andrew Pérez, Valentín Ortega-Mora, Luis Miguel |
author_sort | Arranz-Solís, David |
collection | PubMed |
description | Neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. In order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at days 40, 90 or 120 of gestation. Infection during the first term resulted in the death of all foetuses between days 19 and 21 post-infection, showing mainly necrotic lesions in foetal liver and the highest parasite DNA detection and burden in both placenta and foetal viscera. After infection at day 90, foetal death was also detected in all ewes, although later (34–48 days post-infection). In this group, lesions were mainly inflammatory. Foetal livers showed the lowest frequency of lesions, as well as the lowest parasite detection and burden. All ewes infected at day 120 delivered viable lambs, although 3 out of 9 showed weakness and recumbency. Neospora DNA was detected in all lambs but one, and parasite burden was similar to that observed in day 90 group. Lesions in this group showed more conspicuous infiltration of inflammatory cells and higher frequency in foetal brain and muscle when compared to both previous groups. These results highlight the crucial role that the stage of gestation plays on the course of ovine neosporosis, similar to that reported in bovine neosporosis, and open the doors to consider sheep as a valid model for exogenous transplacental transmission for ruminant neosporosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-014-0139-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4346111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-43461112015-03-03 Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis Arranz-Solís, David Benavides, Julio Regidor-Cerrillo, Javier Fuertes, Miguel Ferre, Ignacio Ferreras, Maria del Carmen Collantes-Fernández, Esther Hemphill, Andrew Pérez, Valentín Ortega-Mora, Luis Miguel Vet Res Research Neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. In order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at days 40, 90 or 120 of gestation. Infection during the first term resulted in the death of all foetuses between days 19 and 21 post-infection, showing mainly necrotic lesions in foetal liver and the highest parasite DNA detection and burden in both placenta and foetal viscera. After infection at day 90, foetal death was also detected in all ewes, although later (34–48 days post-infection). In this group, lesions were mainly inflammatory. Foetal livers showed the lowest frequency of lesions, as well as the lowest parasite detection and burden. All ewes infected at day 120 delivered viable lambs, although 3 out of 9 showed weakness and recumbency. Neospora DNA was detected in all lambs but one, and parasite burden was similar to that observed in day 90 group. Lesions in this group showed more conspicuous infiltration of inflammatory cells and higher frequency in foetal brain and muscle when compared to both previous groups. These results highlight the crucial role that the stage of gestation plays on the course of ovine neosporosis, similar to that reported in bovine neosporosis, and open the doors to consider sheep as a valid model for exogenous transplacental transmission for ruminant neosporosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-014-0139-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-03 2015 /pmc/articles/PMC4346111/ /pubmed/25884945 http://dx.doi.org/10.1186/s13567-014-0139-y Text en © Arranz-Solís et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Arranz-Solís, David Benavides, Julio Regidor-Cerrillo, Javier Fuertes, Miguel Ferre, Ignacio Ferreras, Maria del Carmen Collantes-Fernández, Esther Hemphill, Andrew Pérez, Valentín Ortega-Mora, Luis Miguel Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
title | Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
title_full | Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
title_fullStr | Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
title_full_unstemmed | Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
title_short | Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
title_sort | influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346111/ https://www.ncbi.nlm.nih.gov/pubmed/25884945 http://dx.doi.org/10.1186/s13567-014-0139-y |
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