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Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis

Neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. In order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6...

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Autores principales: Arranz-Solís, David, Benavides, Julio, Regidor-Cerrillo, Javier, Fuertes, Miguel, Ferre, Ignacio, Ferreras, Maria del Carmen, Collantes-Fernández, Esther, Hemphill, Andrew, Pérez, Valentín, Ortega-Mora, Luis Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346111/
https://www.ncbi.nlm.nih.gov/pubmed/25884945
http://dx.doi.org/10.1186/s13567-014-0139-y
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author Arranz-Solís, David
Benavides, Julio
Regidor-Cerrillo, Javier
Fuertes, Miguel
Ferre, Ignacio
Ferreras, Maria del Carmen
Collantes-Fernández, Esther
Hemphill, Andrew
Pérez, Valentín
Ortega-Mora, Luis Miguel
author_facet Arranz-Solís, David
Benavides, Julio
Regidor-Cerrillo, Javier
Fuertes, Miguel
Ferre, Ignacio
Ferreras, Maria del Carmen
Collantes-Fernández, Esther
Hemphill, Andrew
Pérez, Valentín
Ortega-Mora, Luis Miguel
author_sort Arranz-Solís, David
collection PubMed
description Neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. In order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at days 40, 90 or 120 of gestation. Infection during the first term resulted in the death of all foetuses between days 19 and 21 post-infection, showing mainly necrotic lesions in foetal liver and the highest parasite DNA detection and burden in both placenta and foetal viscera. After infection at day 90, foetal death was also detected in all ewes, although later (34–48 days post-infection). In this group, lesions were mainly inflammatory. Foetal livers showed the lowest frequency of lesions, as well as the lowest parasite detection and burden. All ewes infected at day 120 delivered viable lambs, although 3 out of 9 showed weakness and recumbency. Neospora DNA was detected in all lambs but one, and parasite burden was similar to that observed in day 90 group. Lesions in this group showed more conspicuous infiltration of inflammatory cells and higher frequency in foetal brain and muscle when compared to both previous groups. These results highlight the crucial role that the stage of gestation plays on the course of ovine neosporosis, similar to that reported in bovine neosporosis, and open the doors to consider sheep as a valid model for exogenous transplacental transmission for ruminant neosporosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-014-0139-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-43461112015-03-03 Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis Arranz-Solís, David Benavides, Julio Regidor-Cerrillo, Javier Fuertes, Miguel Ferre, Ignacio Ferreras, Maria del Carmen Collantes-Fernández, Esther Hemphill, Andrew Pérez, Valentín Ortega-Mora, Luis Miguel Vet Res Research Neospora caninum is considered one of the main causes of abortion in cattle, yet recent studies have also emphasised its relevance as an abortifacient in small ruminants. In order to gain deeper insight into the pathogenesis of ovine neosporosis, pregnant ewes were intravenously inoculated with 10(6) tachyzoites of the Nc-Spain7 isolate at days 40, 90 or 120 of gestation. Infection during the first term resulted in the death of all foetuses between days 19 and 21 post-infection, showing mainly necrotic lesions in foetal liver and the highest parasite DNA detection and burden in both placenta and foetal viscera. After infection at day 90, foetal death was also detected in all ewes, although later (34–48 days post-infection). In this group, lesions were mainly inflammatory. Foetal livers showed the lowest frequency of lesions, as well as the lowest parasite detection and burden. All ewes infected at day 120 delivered viable lambs, although 3 out of 9 showed weakness and recumbency. Neospora DNA was detected in all lambs but one, and parasite burden was similar to that observed in day 90 group. Lesions in this group showed more conspicuous infiltration of inflammatory cells and higher frequency in foetal brain and muscle when compared to both previous groups. These results highlight the crucial role that the stage of gestation plays on the course of ovine neosporosis, similar to that reported in bovine neosporosis, and open the doors to consider sheep as a valid model for exogenous transplacental transmission for ruminant neosporosis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13567-014-0139-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-03-03 2015 /pmc/articles/PMC4346111/ /pubmed/25884945 http://dx.doi.org/10.1186/s13567-014-0139-y Text en © Arranz-Solís et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Arranz-Solís, David
Benavides, Julio
Regidor-Cerrillo, Javier
Fuertes, Miguel
Ferre, Ignacio
Ferreras, Maria del Carmen
Collantes-Fernández, Esther
Hemphill, Andrew
Pérez, Valentín
Ortega-Mora, Luis Miguel
Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
title Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
title_full Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
title_fullStr Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
title_full_unstemmed Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
title_short Influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
title_sort influence of the gestational stage on the clinical course, lesional development and parasite distribution in experimental ovine neosporosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346111/
https://www.ncbi.nlm.nih.gov/pubmed/25884945
http://dx.doi.org/10.1186/s13567-014-0139-y
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