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Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum

Combinatorial effects of influential growth nutrients were investigated in order to enhance hydrogen (H(2)) production during direct conversion of cellulose by Clostridium thermocellum DSM 1237. A central composite face-centered design and response surface methodology (RSM) were applied to optimize...

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Autores principales: Islam, Rumana, Sparling, Richard, Cicek, Nazim, Levin, David B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346883/
https://www.ncbi.nlm.nih.gov/pubmed/25647413
http://dx.doi.org/10.3390/ijms16023116
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author Islam, Rumana
Sparling, Richard
Cicek, Nazim
Levin, David B.
author_facet Islam, Rumana
Sparling, Richard
Cicek, Nazim
Levin, David B.
author_sort Islam, Rumana
collection PubMed
description Combinatorial effects of influential growth nutrients were investigated in order to enhance hydrogen (H(2)) production during direct conversion of cellulose by Clostridium thermocellum DSM 1237. A central composite face-centered design and response surface methodology (RSM) were applied to optimize concentrations of cellulose, yeast extract (YE), and magnesium chloride (Mg) in culture. The overall optimum composition generated by the desirability function resulted in 57.28 mmol H(2)/L-culture with 1.30 mol H(2)/mol glucose and 7.48 mmol/(g·cell·h) when cultures contained 25 g/L cellulose, 2 g/L YE, and 1.75 g/L Mg. Compared with the unaltered medium, the optimized medium produced approximately 3.2-fold more H(2) within the same time-frame with 50% higher specific productivity, which are also better than previously reported values from similar studies. Nutrient composition that diverted carbon and electron flux away from H(2) promoting ethanol production was also determined. This study represents the first investigation dealing with multifactor optimization with RSM for H(2) production during direct cellulose fermentation.
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spelling pubmed-43468832015-04-03 Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum Islam, Rumana Sparling, Richard Cicek, Nazim Levin, David B. Int J Mol Sci Article Combinatorial effects of influential growth nutrients were investigated in order to enhance hydrogen (H(2)) production during direct conversion of cellulose by Clostridium thermocellum DSM 1237. A central composite face-centered design and response surface methodology (RSM) were applied to optimize concentrations of cellulose, yeast extract (YE), and magnesium chloride (Mg) in culture. The overall optimum composition generated by the desirability function resulted in 57.28 mmol H(2)/L-culture with 1.30 mol H(2)/mol glucose and 7.48 mmol/(g·cell·h) when cultures contained 25 g/L cellulose, 2 g/L YE, and 1.75 g/L Mg. Compared with the unaltered medium, the optimized medium produced approximately 3.2-fold more H(2) within the same time-frame with 50% higher specific productivity, which are also better than previously reported values from similar studies. Nutrient composition that diverted carbon and electron flux away from H(2) promoting ethanol production was also determined. This study represents the first investigation dealing with multifactor optimization with RSM for H(2) production during direct cellulose fermentation. MDPI 2015-01-30 /pmc/articles/PMC4346883/ /pubmed/25647413 http://dx.doi.org/10.3390/ijms16023116 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Islam, Rumana
Sparling, Richard
Cicek, Nazim
Levin, David B.
Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum
title Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum
title_full Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum
title_fullStr Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum
title_full_unstemmed Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum
title_short Optimization of Influential Nutrients during Direct Cellulose Fermentation into Hydrogen by Clostridium thermocellum
title_sort optimization of influential nutrients during direct cellulose fermentation into hydrogen by clostridium thermocellum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346883/
https://www.ncbi.nlm.nih.gov/pubmed/25647413
http://dx.doi.org/10.3390/ijms16023116
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