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Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces

Peri-implant infections from bacterial biofilms on artificial surfaces are a common threat to all medical implants. They are a handicap for the patient and can lead to implant failure or even life-threatening complications. New implant surfaces have to be developed to reduce biofilm formation and to...

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Autores principales: Winkel, Andreas, Dempwolf, Wibke, Gellermann, Eva, Sluszniak, Magdalena, Grade, Sebastian, Heuer, Wieland, Eisenburger, Michael, Menzel, Henning, Stiesch, Meike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346959/
https://www.ncbi.nlm.nih.gov/pubmed/25690041
http://dx.doi.org/10.3390/ijms16024327
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author Winkel, Andreas
Dempwolf, Wibke
Gellermann, Eva
Sluszniak, Magdalena
Grade, Sebastian
Heuer, Wieland
Eisenburger, Michael
Menzel, Henning
Stiesch, Meike
author_facet Winkel, Andreas
Dempwolf, Wibke
Gellermann, Eva
Sluszniak, Magdalena
Grade, Sebastian
Heuer, Wieland
Eisenburger, Michael
Menzel, Henning
Stiesch, Meike
author_sort Winkel, Andreas
collection PubMed
description Peri-implant infections from bacterial biofilms on artificial surfaces are a common threat to all medical implants. They are a handicap for the patient and can lead to implant failure or even life-threatening complications. New implant surfaces have to be developed to reduce biofilm formation and to improve the long-term prognosis of medical implants. The aim of this study was (1) to develop a new method to test the antibacterial efficacy of implant surfaces by direct surface contact and (2) to elucidate whether an innovative antimicrobial copolymer coating of 4-vinyl-N-hexylpyridinium bromide and dimethyl(2-methacryloyloxyethyl) phosphonate (VP:DMMEP 30:70) on titanium is able to reduce the attachment of bacteria prevalent in peri-implant infections. With a new in vitro model with semi-coated titanium discs, we were able to show a dramatic reduction in the adhesion of various pathogenic bacteria (Streptococcus sanguinis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis), completely independently of effects caused by soluble materials. In contrast, soft tissue cells (human gingival or dermis fibroblasts) were less affected by the same coating, despite a moderate reduction in initial adhesion of gingival fibroblasts. These data confirm the hypothesis that VP:DMMEP 30:70 is a promising antibacterial copolymer that may be of use in several clinical applications.
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spelling pubmed-43469592015-04-03 Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces Winkel, Andreas Dempwolf, Wibke Gellermann, Eva Sluszniak, Magdalena Grade, Sebastian Heuer, Wieland Eisenburger, Michael Menzel, Henning Stiesch, Meike Int J Mol Sci Article Peri-implant infections from bacterial biofilms on artificial surfaces are a common threat to all medical implants. They are a handicap for the patient and can lead to implant failure or even life-threatening complications. New implant surfaces have to be developed to reduce biofilm formation and to improve the long-term prognosis of medical implants. The aim of this study was (1) to develop a new method to test the antibacterial efficacy of implant surfaces by direct surface contact and (2) to elucidate whether an innovative antimicrobial copolymer coating of 4-vinyl-N-hexylpyridinium bromide and dimethyl(2-methacryloyloxyethyl) phosphonate (VP:DMMEP 30:70) on titanium is able to reduce the attachment of bacteria prevalent in peri-implant infections. With a new in vitro model with semi-coated titanium discs, we were able to show a dramatic reduction in the adhesion of various pathogenic bacteria (Streptococcus sanguinis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis), completely independently of effects caused by soluble materials. In contrast, soft tissue cells (human gingival or dermis fibroblasts) were less affected by the same coating, despite a moderate reduction in initial adhesion of gingival fibroblasts. These data confirm the hypothesis that VP:DMMEP 30:70 is a promising antibacterial copolymer that may be of use in several clinical applications. MDPI 2015-02-17 /pmc/articles/PMC4346959/ /pubmed/25690041 http://dx.doi.org/10.3390/ijms16024327 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Winkel, Andreas
Dempwolf, Wibke
Gellermann, Eva
Sluszniak, Magdalena
Grade, Sebastian
Heuer, Wieland
Eisenburger, Michael
Menzel, Henning
Stiesch, Meike
Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces
title Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces
title_full Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces
title_fullStr Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces
title_full_unstemmed Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces
title_short Introducing a Semi-Coated Model to Investigate Antibacterial Effects of Biocompatible Polymers on Titanium Surfaces
title_sort introducing a semi-coated model to investigate antibacterial effects of biocompatible polymers on titanium surfaces
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346959/
https://www.ncbi.nlm.nih.gov/pubmed/25690041
http://dx.doi.org/10.3390/ijms16024327
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