Establishment of primary cultures of craniopharyngioma cells★

Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 p...

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Autores principales: Liu, Hao, Liu, Liang, Liu, Zhiyong, Li, Qiang, You, Chao, Xu, Jianguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Technique and Method: Brain Injury and Neuroregeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346985/
https://www.ncbi.nlm.nih.gov/pubmed/25745451
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.08.007
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author Liu, Hao
Liu, Liang
Liu, Zhiyong
Li, Qiang
You, Chao
Xu, Jianguo
author_facet Liu, Hao
Liu, Liang
Liu, Zhiyong
Li, Qiang
You, Chao
Xu, Jianguo
author_sort Liu, Hao
collection PubMed
description Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 passages, confirming that all cultured cells were squamous epithelial cells. The doubling time of craniopharyngioma cells was 3 days, as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In this study, craniopharyngioma cells cultured in vitro were established; however, establishment of immortalized craniopharyngioma cell lines requires further research.
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spelling pubmed-43469852015-03-05 Establishment of primary cultures of craniopharyngioma cells★ Liu, Hao Liu, Liang Liu, Zhiyong Li, Qiang You, Chao Xu, Jianguo Neural Regen Res Technique and Method: Brain Injury and Neuroregeneration Craniopharynigoma samples were collected from 36 patients. Out of the 36 samples, 29 achieved successful sub-culturing, with a success rate of 80.6%. Immunohistochemistry staining showed that cytokeratin-7 was positively expressed in the cytomembrane and cytoplasm of craniopharyngioma cells at 6-8 passages, confirming that all cultured cells were squamous epithelial cells. The doubling time of craniopharyngioma cells was 3 days, as confirmed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In this study, craniopharyngioma cells cultured in vitro were established; however, establishment of immortalized craniopharyngioma cell lines requires further research. Medknow Publications & Media Pvt Ltd 2012-03-15 /pmc/articles/PMC4346985/ /pubmed/25745451 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.08.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technique and Method: Brain Injury and Neuroregeneration
Liu, Hao
Liu, Liang
Liu, Zhiyong
Li, Qiang
You, Chao
Xu, Jianguo
Establishment of primary cultures of craniopharyngioma cells★
title Establishment of primary cultures of craniopharyngioma cells★
title_full Establishment of primary cultures of craniopharyngioma cells★
title_fullStr Establishment of primary cultures of craniopharyngioma cells★
title_full_unstemmed Establishment of primary cultures of craniopharyngioma cells★
title_short Establishment of primary cultures of craniopharyngioma cells★
title_sort establishment of primary cultures of craniopharyngioma cells★
topic Technique and Method: Brain Injury and Neuroregeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4346985/
https://www.ncbi.nlm.nih.gov/pubmed/25745451
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.08.007
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