Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆

Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were...

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Autores principales: Ruan, Xiyun, Yuan, Zhenguo, Du, Yifeng, Yang, Guangxiao, Wang, Quanying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Techniques and Method: Emerging Technology in Neuroregeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347013/
https://www.ncbi.nlm.nih.gov/pubmed/25745468
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.012
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author Ruan, Xiyun
Yuan, Zhenguo
Du, Yifeng
Yang, Guangxiao
Wang, Quanying
author_facet Ruan, Xiyun
Yuan, Zhenguo
Du, Yifeng
Yang, Guangxiao
Wang, Quanying
author_sort Ruan, Xiyun
collection PubMed
description Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.
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spelling pubmed-43470132015-03-05 Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆ Ruan, Xiyun Yuan, Zhenguo Du, Yifeng Yang, Guangxiao Wang, Quanying Neural Regen Res Techniques and Method: Emerging Technology in Neuroregeneration Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells. Medknow Publications & Media Pvt Ltd 2012-03-25 /pmc/articles/PMC4347013/ /pubmed/25745468 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.012 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Techniques and Method: Emerging Technology in Neuroregeneration
Ruan, Xiyun
Yuan, Zhenguo
Du, Yifeng
Yang, Guangxiao
Wang, Quanying
Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆
title Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆
title_full Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆
title_fullStr Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆
title_full_unstemmed Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆
title_short Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells☆
title_sort recombinant adeno-associated virus delivered human thioredoxin-pr39 prevents hypoxia-induced apoptosis of ecv304 cells☆
topic Techniques and Method: Emerging Technology in Neuroregeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347013/
https://www.ncbi.nlm.nih.gov/pubmed/25745468
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.09.012
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