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Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model
PURPOSE: Intimal hyperplasia (IH) is the main cause of restenosis or occlusion after vascular procedures. Imatinib mesylate and rapamycin are known to prevent IH. The purpose of this study was to evaluate the effect of these drugs on the regression of preformed IH in rat carotid injury model. METHOD...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Surgical Society
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347041/ https://www.ncbi.nlm.nih.gov/pubmed/25741495 http://dx.doi.org/10.4174/astr.2015.88.3.152 |
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author | Park, Yang Jin Min, Seung-Kee Min, Sang-Il Kim, Sang Joon Ha, Jongwon |
author_facet | Park, Yang Jin Min, Seung-Kee Min, Sang-Il Kim, Sang Joon Ha, Jongwon |
author_sort | Park, Yang Jin |
collection | PubMed |
description | PURPOSE: Intimal hyperplasia (IH) is the main cause of restenosis or occlusion after vascular procedures. Imatinib mesylate and rapamycin are known to prevent IH. The purpose of this study was to evaluate the effect of these drugs on the regression of preformed IH in rat carotid injury model. METHODS: IH was established in rat carotid arteries using a balloon catheter. The drug effects were assessed in vitro on proliferation, migration, and apoptosis of vascular smooth muscle cells (VSMC) in the neointima. And in vivo studies were carried out in 4 groups: imatinib, rapamycin, combined, and no medication. After 2-week oral medication, morphometric analysis evaluated the number and density of neointimal cells, intima-to-media (I/M) ratio and cross-sectional area. Cell proliferation, apoptosis, and collagen changes were also investigated by immunohistochemical staining (IHCS). RESULTS: Imatinib and rapamycin significantly inhibited VSMC proliferation and migration, and promoted apoptosis in vitro. In morphometric analysis, the number and density of neointimal cells decreased significantly in all medication groups compared with control group (P < 0.01). However, there was no significant difference in neointimal cross-sectional area and I/M ratio among groups. In IHCS, imatinib and rapamycin inhibited neointimal cell proliferation significantly. However, there was no significant change in cell apoptosis and collagen composition. CONCLUSION: Combined treatment of with imatinib and rapamycin induced reduction of cell mass in preformed intimal hyperplasia, but failed to induce regression of intimal mass in this short-term medication study. Further studies will be needed with additional strategies of inducing lysis of the extracellular matrix. |
format | Online Article Text |
id | pubmed-4347041 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The Korean Surgical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-43470412015-03-04 Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model Park, Yang Jin Min, Seung-Kee Min, Sang-Il Kim, Sang Joon Ha, Jongwon Ann Surg Treat Res Original Article PURPOSE: Intimal hyperplasia (IH) is the main cause of restenosis or occlusion after vascular procedures. Imatinib mesylate and rapamycin are known to prevent IH. The purpose of this study was to evaluate the effect of these drugs on the regression of preformed IH in rat carotid injury model. METHODS: IH was established in rat carotid arteries using a balloon catheter. The drug effects were assessed in vitro on proliferation, migration, and apoptosis of vascular smooth muscle cells (VSMC) in the neointima. And in vivo studies were carried out in 4 groups: imatinib, rapamycin, combined, and no medication. After 2-week oral medication, morphometric analysis evaluated the number and density of neointimal cells, intima-to-media (I/M) ratio and cross-sectional area. Cell proliferation, apoptosis, and collagen changes were also investigated by immunohistochemical staining (IHCS). RESULTS: Imatinib and rapamycin significantly inhibited VSMC proliferation and migration, and promoted apoptosis in vitro. In morphometric analysis, the number and density of neointimal cells decreased significantly in all medication groups compared with control group (P < 0.01). However, there was no significant difference in neointimal cross-sectional area and I/M ratio among groups. In IHCS, imatinib and rapamycin inhibited neointimal cell proliferation significantly. However, there was no significant change in cell apoptosis and collagen composition. CONCLUSION: Combined treatment of with imatinib and rapamycin induced reduction of cell mass in preformed intimal hyperplasia, but failed to induce regression of intimal mass in this short-term medication study. Further studies will be needed with additional strategies of inducing lysis of the extracellular matrix. The Korean Surgical Society 2015-03 2015-02-27 /pmc/articles/PMC4347041/ /pubmed/25741495 http://dx.doi.org/10.4174/astr.2015.88.3.152 Text en Copyright © 2015, the Korean Surgical Society http://creativecommons.org/licenses/by-nc/3.0/ Annals of Surgical Treatment and Research is an Open Access Journal. All articles are distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Park, Yang Jin Min, Seung-Kee Min, Sang-Il Kim, Sang Joon Ha, Jongwon Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
title | Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
title_full | Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
title_fullStr | Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
title_full_unstemmed | Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
title_short | Effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
title_sort | effect of imatinib mesylate and rapamycin on the preformed intimal hyperplasia in rat carotid injury model |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4347041/ https://www.ncbi.nlm.nih.gov/pubmed/25741495 http://dx.doi.org/10.4174/astr.2015.88.3.152 |
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