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Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆

This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatme...

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Autores principales: Yu, Lingli, Ding, Yindi, Spencer, Ambre, Ma, Ji, Lu, Ruisheng, Rudkin, Brian B., Yuan, Chonggang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348992/
https://www.ncbi.nlm.nih.gov/pubmed/25745432
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.001
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author Yu, Lingli
Ding, Yindi
Spencer, Ambre
Ma, Ji
Lu, Ruisheng
Rudkin, Brian B.
Yuan, Chonggang
author_facet Yu, Lingli
Ding, Yindi
Spencer, Ambre
Ma, Ji
Lu, Ruisheng
Rudkin, Brian B.
Yuan, Chonggang
author_sort Yu, Lingli
collection PubMed
description This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells.
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spelling pubmed-43489922015-03-05 Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆ Yu, Lingli Ding, Yindi Spencer, Ambre Ma, Ji Lu, Ruisheng Rudkin, Brian B. Yuan, Chonggang Neural Regen Res Research and Report Article: Neurogenesis and Neural Plasticity This study examined the isolation and differentiation of dorsal root ganglion progenitor cells for therapeutic use in neurodegenerative diseases. Rat embryonic dorsal root ganglia progenitors were isolated and purified using the differential adhesion method combined with cytosine arabinoside treatment. After culture in serum-free medium supplemented with B27, basic fibroblast growth factor and epidermal growth factor, these cells remained viable and survived for more than 18 months in vitro. Most cells differentiated to neurons that were immunoreactive for gamma-aminobutyric acid and choline acetyltransferase as detected by immunohistochemical staining. In addition, nerve growth factor and neurotrophic tyrosine kinase receptor expression were also observed in dorsal root ganglion progenitors and differentiated cells. K252a, an inhibitor that blocks nerve growth factor-induced signaling, inhibited cell survival, suggesting the possible existence of a nerve growth factor autocrine loop in these proliferating cells. Medknow Publications & Media Pvt Ltd 2012-03-05 /pmc/articles/PMC4348992/ /pubmed/25745432 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.001 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report Article: Neurogenesis and Neural Plasticity
Yu, Lingli
Ding, Yindi
Spencer, Ambre
Ma, Ji
Lu, Ruisheng
Rudkin, Brian B.
Yuan, Chonggang
Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
title Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
title_full Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
title_fullStr Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
title_full_unstemmed Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
title_short Dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
title_sort dorsal root ganglion progenitors differentiate to gamma-aminobutyric acid- and choline acetyltransferase-positive neurons☆
topic Research and Report Article: Neurogenesis and Neural Plasticity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348992/
https://www.ncbi.nlm.nih.gov/pubmed/25745432
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.001
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