Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★

Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significant...

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Detalles Bibliográficos
Autores principales: Chen, Jing, Hao, Hu, Guo, Guoqing, Li, Sitao, Xiao, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report Article: Neurogenesis and Neural Plasticity
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348994/
https://www.ncbi.nlm.nih.gov/pubmed/25745434
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.003
Descripción
Sumario:Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons.

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