Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★

Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significant...

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Autores principales: Chen, Jing, Hao, Hu, Guo, Guoqing, Li, Sitao, Xiao, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report Article: Neurogenesis and Neural Plasticity
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348994/
https://www.ncbi.nlm.nih.gov/pubmed/25745434
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.003
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author Chen, Jing
Hao, Hu
Guo, Guoqing
Li, Sitao
Xiao, Xin
author_facet Chen, Jing
Hao, Hu
Guo, Guoqing
Li, Sitao
Xiao, Xin
author_sort Chen, Jing
collection PubMed
description Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons.
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spelling pubmed-43489942015-03-05 Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★ Chen, Jing Hao, Hu Guo, Guoqing Li, Sitao Xiao, Xin Neural Regen Res Research and Report Article: Neurogenesis and Neural Plasticity Hippocampal neurons of neonatal rats were cultured in serum-free culture medium for 5 days in vitro, and treated with the Rho-kinase inducer lysophosphatidic acid. Atomic force microscopy revealed that the numbers of level-1, -2 and -3 neurites protruding from rat hippocampal neurons was significantly reduced. After treatment with the Rho kinase inhibitor Y27632, a significant increase in the numbers of these neurites was observed. Our experimental findings indicate that the Rho-kinase pathway is closely associated with the neurites of hippocampal neurons. Medknow Publications & Media Pvt Ltd 2012-03-05 /pmc/articles/PMC4348994/ /pubmed/25745434 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.003 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report Article: Neurogenesis and Neural Plasticity
Chen, Jing
Hao, Hu
Guo, Guoqing
Li, Sitao
Xiao, Xin
Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
title Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
title_full Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
title_fullStr Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
title_full_unstemmed Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
title_short Effect of Rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
title_sort effect of rho-kinase pathway on neurite outgrowth of rat hippocampal neurons under atomic force microscopy★
topic Research and Report Article: Neurogenesis and Neural Plasticity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4348994/
https://www.ncbi.nlm.nih.gov/pubmed/25745434
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.07.003
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