Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains

Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of diff...

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Detalles Bibliográficos
Autores principales: Chen, Jun-Song, Beckley, Janel R., McDonald, Nathan A., Ren, Liping, Mangione, MariaSanta, Jang, Sylvia J., Elmore, Zachary C., Rachfall, Nicole, Feoktistova, Anna, Jones, Christine M., Willet, Alaina H., Guillen, Rodrigo, Bitton, Danny A., Bähler, Jürg, Jensen, Michael A., Rhind, Nick, Gould, Kathleen L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349090/
https://www.ncbi.nlm.nih.gov/pubmed/25552606
http://dx.doi.org/10.1534/g3.114.015701
Descripción
Sumario:Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.

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