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Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains
Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of diff...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Genetics Society of America
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349090/ https://www.ncbi.nlm.nih.gov/pubmed/25552606 http://dx.doi.org/10.1534/g3.114.015701 |
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| author | Chen, Jun-Song Beckley, Janel R. McDonald, Nathan A. Ren, Liping Mangione, MariaSanta Jang, Sylvia J. Elmore, Zachary C. Rachfall, Nicole Feoktistova, Anna Jones, Christine M. Willet, Alaina H. Guillen, Rodrigo Bitton, Danny A. Bähler, Jürg Jensen, Michael A. Rhind, Nick Gould, Kathleen L. |
| author_facet | Chen, Jun-Song Beckley, Janel R. McDonald, Nathan A. Ren, Liping Mangione, MariaSanta Jang, Sylvia J. Elmore, Zachary C. Rachfall, Nicole Feoktistova, Anna Jones, Christine M. Willet, Alaina H. Guillen, Rodrigo Bitton, Danny A. Bähler, Jürg Jensen, Michael A. Rhind, Nick Gould, Kathleen L. |
| author_sort | Chen, Jun-Song |
| collection | PubMed |
| description | Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe. |
| format | Online Article Text |
| id | pubmed-4349090 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| publisher | Genetics Society of America |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-43490902015-03-11 Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains Chen, Jun-Song Beckley, Janel R. McDonald, Nathan A. Ren, Liping Mangione, MariaSanta Jang, Sylvia J. Elmore, Zachary C. Rachfall, Nicole Feoktistova, Anna Jones, Christine M. Willet, Alaina H. Guillen, Rodrigo Bitton, Danny A. Bähler, Jürg Jensen, Michael A. Rhind, Nick Gould, Kathleen L. G3 (Bethesda) Investigations Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe. Genetics Society of America 2014-12-31 /pmc/articles/PMC4349090/ /pubmed/25552606 http://dx.doi.org/10.1534/g3.114.015701 Text en Copyright © 2015 Chen et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Investigations Chen, Jun-Song Beckley, Janel R. McDonald, Nathan A. Ren, Liping Mangione, MariaSanta Jang, Sylvia J. Elmore, Zachary C. Rachfall, Nicole Feoktistova, Anna Jones, Christine M. Willet, Alaina H. Guillen, Rodrigo Bitton, Danny A. Bähler, Jürg Jensen, Michael A. Rhind, Nick Gould, Kathleen L. Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
| title | Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
| title_full | Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
| title_fullStr | Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
| title_full_unstemmed | Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
| title_short | Identification of New Players in Cell Division, DNA Damage Response, and Morphogenesis Through Construction of Schizosaccharomyces pombe Deletion Strains |
| title_sort | identification of new players in cell division, dna damage response, and morphogenesis through construction of schizosaccharomyces pombe deletion strains |
| topic | Investigations |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349090/ https://www.ncbi.nlm.nih.gov/pubmed/25552606 http://dx.doi.org/10.1534/g3.114.015701 |
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