Genome engineering and direct cloning of antibiotic gene clusters via phage ϕBT1 integrase-mediated site-specific recombination in Streptomyces

Several strategies have been used to clone large DNA fragments directly from bacterial genome. Most of these approaches are based on different site-specific recombination systems consisting of a specialized recombinase and its target sites. In this study, a novel strategy based on phage ϕBT1 integra...

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Detalles Bibliográficos
Autores principales: Du, Deyao, Wang, Lu, Tian, Yuqing, Liu, Hao, Tan, Huarong, Niu, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349145/
https://www.ncbi.nlm.nih.gov/pubmed/25737113
http://dx.doi.org/10.1038/srep08740

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