Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo

Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in...

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Autores principales: Zanette, Régis A., Bitencourt, Paula E. R., Kontoyiannis, Dimitrios P., Fighera, Rafael A., Flores, Mariana M., Kommers, Glaucia D., Silva, Priscila S., Ludwig, Aline, Moretto, Maria B., Alves, Sydney H., Santurio, Janio M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349436/
https://www.ncbi.nlm.nih.gov/pubmed/25738758
http://dx.doi.org/10.1371/journal.pone.0118932
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author Zanette, Régis A.
Bitencourt, Paula E. R.
Kontoyiannis, Dimitrios P.
Fighera, Rafael A.
Flores, Mariana M.
Kommers, Glaucia D.
Silva, Priscila S.
Ludwig, Aline
Moretto, Maria B.
Alves, Sydney H.
Santurio, Janio M.
author_facet Zanette, Régis A.
Bitencourt, Paula E. R.
Kontoyiannis, Dimitrios P.
Fighera, Rafael A.
Flores, Mariana M.
Kommers, Glaucia D.
Silva, Priscila S.
Ludwig, Aline
Moretto, Maria B.
Alves, Sydney H.
Santurio, Janio M.
author_sort Zanette, Régis A.
collection PubMed
description Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in the presence of exogenous iron. The tissue activity of the proinflammatory enzyme adenosine deaminase and the histological pattern observed in pythiosis lesions of rabbits treated with deferasirox were similar to the ones in animals treated with immunotherapy.
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spelling pubmed-43494362015-03-17 Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo Zanette, Régis A. Bitencourt, Paula E. R. Kontoyiannis, Dimitrios P. Fighera, Rafael A. Flores, Mariana M. Kommers, Glaucia D. Silva, Priscila S. Ludwig, Aline Moretto, Maria B. Alves, Sydney H. Santurio, Janio M. PLoS One Research Article Pythium insidiosum iron acquisition mechanisms are unknown. We previously showed that the iron chelator deferasirox had weak activity in vitro and in rabbits with experimental pythiosis. Here we show that deferasirox causes damage to P. insidiosum hyphae in vitro, but that activity is diminished in the presence of exogenous iron. The tissue activity of the proinflammatory enzyme adenosine deaminase and the histological pattern observed in pythiosis lesions of rabbits treated with deferasirox were similar to the ones in animals treated with immunotherapy. Public Library of Science 2015-03-04 /pmc/articles/PMC4349436/ /pubmed/25738758 http://dx.doi.org/10.1371/journal.pone.0118932 Text en © 2015 Zanette et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zanette, Régis A.
Bitencourt, Paula E. R.
Kontoyiannis, Dimitrios P.
Fighera, Rafael A.
Flores, Mariana M.
Kommers, Glaucia D.
Silva, Priscila S.
Ludwig, Aline
Moretto, Maria B.
Alves, Sydney H.
Santurio, Janio M.
Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo
title Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo
title_full Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo
title_fullStr Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo
title_full_unstemmed Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo
title_short Complex Interaction of Deferasirox and Pythium insidiosum: Iron-Dependent Attenuation of Growth In Vitro and Immunotherapy-Like Enhancement of Immune Responses In Vivo
title_sort complex interaction of deferasirox and pythium insidiosum: iron-dependent attenuation of growth in vitro and immunotherapy-like enhancement of immune responses in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349436/
https://www.ncbi.nlm.nih.gov/pubmed/25738758
http://dx.doi.org/10.1371/journal.pone.0118932
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