Cloning and expression analysis of prohibitin mRNA in canine mammary tumors

Prohibitin is an antiproliferative protein that is a product of a putative tumor suppressor gene. However, there is little information on prohibitins in companion animals. In this study, we cloned canine prohibitin mRNA using RT-PCR and 3′-RACE (Rapid Amplification of cDNA Ends). The sequence was we...

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Autores principales: MATSUYAMA, Satoshi, NAKANO, Yuko, NAKAMURA, Mieko, YAMAMOTO, Ryohei, SHIMADA, Terumasa, OHASHI, Fumihito, KUBO, Kihei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Surgery
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349545/
https://www.ncbi.nlm.nih.gov/pubmed/25312047
http://dx.doi.org/10.1292/jvms.14-0239
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author MATSUYAMA, Satoshi
NAKANO, Yuko
NAKAMURA, Mieko
YAMAMOTO, Ryohei
SHIMADA, Terumasa
OHASHI, Fumihito
KUBO, Kihei
author_facet MATSUYAMA, Satoshi
NAKANO, Yuko
NAKAMURA, Mieko
YAMAMOTO, Ryohei
SHIMADA, Terumasa
OHASHI, Fumihito
KUBO, Kihei
author_sort MATSUYAMA, Satoshi
collection PubMed
description Prohibitin is an antiproliferative protein that is a product of a putative tumor suppressor gene. However, there is little information on prohibitins in companion animals. In this study, we cloned canine prohibitin mRNA using RT-PCR and 3′-RACE (Rapid Amplification of cDNA Ends). The sequence was well conserved compared with those of other mammals, including human. The deduced amino acid sequence translated from the open reading frame completely corresponded to the human sequence. Canine prohibitin mRNA was expressed in all normal mammary and tumor samples examined. These results suggest that this protein plays a vital role in cell growth mechanisms and may be related to the occurrence of canine mammary tumors.
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spelling pubmed-43495452015-03-09 Cloning and expression analysis of prohibitin mRNA in canine mammary tumors MATSUYAMA, Satoshi NAKANO, Yuko NAKAMURA, Mieko YAMAMOTO, Ryohei SHIMADA, Terumasa OHASHI, Fumihito KUBO, Kihei J Vet Med Sci Surgery Prohibitin is an antiproliferative protein that is a product of a putative tumor suppressor gene. However, there is little information on prohibitins in companion animals. In this study, we cloned canine prohibitin mRNA using RT-PCR and 3′-RACE (Rapid Amplification of cDNA Ends). The sequence was well conserved compared with those of other mammals, including human. The deduced amino acid sequence translated from the open reading frame completely corresponded to the human sequence. Canine prohibitin mRNA was expressed in all normal mammary and tumor samples examined. These results suggest that this protein plays a vital role in cell growth mechanisms and may be related to the occurrence of canine mammary tumors. The Japanese Society of Veterinary Science 2014-10-14 2015-01 /pmc/articles/PMC4349545/ /pubmed/25312047 http://dx.doi.org/10.1292/jvms.14-0239 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Surgery
MATSUYAMA, Satoshi
NAKANO, Yuko
NAKAMURA, Mieko
YAMAMOTO, Ryohei
SHIMADA, Terumasa
OHASHI, Fumihito
KUBO, Kihei
Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
title Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
title_full Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
title_fullStr Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
title_full_unstemmed Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
title_short Cloning and expression analysis of prohibitin mRNA in canine mammary tumors
title_sort cloning and expression analysis of prohibitin mrna in canine mammary tumors
topic Surgery
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4349545/
https://www.ncbi.nlm.nih.gov/pubmed/25312047
http://dx.doi.org/10.1292/jvms.14-0239
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