Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

The CRISPR/Cas9 system provides a powerful tool for genome editing in various model organisms, including zebrafish. The establishment of targeted gene-disrupted zebrafish (knockouts) is readily achieved by CRISPR/Cas9-mediated genome modification. Recently, exogenous DNA integration into the zebrafi...

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Detalles Bibliográficos
Autores principales: Hisano, Yu, Sakuma, Tetsushi, Nakade, Shota, Ohga, Rie, Ota, Satoshi, Okamoto, Hitoshi, Yamamoto, Takashi, Kawahara, Atsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350073/
https://www.ncbi.nlm.nih.gov/pubmed/25740433
http://dx.doi.org/10.1038/srep08841

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