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Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug
Phytophagous pentatomid insects can negatively impact agricultural productivity and the brown marmorated stink bug (Halyomorpha halys) is an emerging invasive pest responsible for damage to many fruit crops and ornamental plants in North America. Many phytophagous stink bugs, including H. halys, har...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350177/ https://www.ncbi.nlm.nih.gov/pubmed/25587021 http://dx.doi.org/10.1093/gbe/evv006 |
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author | Kenyon, Laura J. Meulia, Tea Sabree, Zakee L. |
author_facet | Kenyon, Laura J. Meulia, Tea Sabree, Zakee L. |
author_sort | Kenyon, Laura J. |
collection | PubMed |
description | Phytophagous pentatomid insects can negatively impact agricultural productivity and the brown marmorated stink bug (Halyomorpha halys) is an emerging invasive pest responsible for damage to many fruit crops and ornamental plants in North America. Many phytophagous stink bugs, including H. halys, harbor gammaproteobacterial symbionts that likely contribute to host development, and characterization of symbiont transmission/acquisition and their contribution to host fitness may offer alternative strategies for managing pest species. “Candidatus Pantoea carbekii” is the primary occupant of gastric ceca lumina flanking the distal midgut of H. halys insects and it is acquired each generation when nymphs feed on maternal extrachorion secretions following hatching. Insects prevented from symbiont uptake exhibit developmental delays and aberrant behaviors. To infer contributions of Ca. P. carbekii to H. halys, the complete genome was sequenced and annotated from a North American H. halys population. Overall, the Ca. P. carbekii genome is nearly one-fourth (1.2 Mb) that of free-living congenerics, and retains genes encoding many functions that are potentially host-supportive. Gene content reflects patterns of gene loss/retention typical of intracellular mutualists of plant-feeding insects. Electron and fluorescence in situ microscopic imaging of H. halys egg surfaces revealed that maternal extrachorion secretions were populated with Ca. P. carbekii cells. The reported findings detail a transgenerational mode of symbiont transmission distinct from that observed for intracellular insect mutualists and illustrate the potential additive functions contributed by the bacterial symbiont to this important agricultural pest. |
format | Online Article Text |
id | pubmed-4350177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-43501772015-03-06 Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug Kenyon, Laura J. Meulia, Tea Sabree, Zakee L. Genome Biol Evol Research Article Phytophagous pentatomid insects can negatively impact agricultural productivity and the brown marmorated stink bug (Halyomorpha halys) is an emerging invasive pest responsible for damage to many fruit crops and ornamental plants in North America. Many phytophagous stink bugs, including H. halys, harbor gammaproteobacterial symbionts that likely contribute to host development, and characterization of symbiont transmission/acquisition and their contribution to host fitness may offer alternative strategies for managing pest species. “Candidatus Pantoea carbekii” is the primary occupant of gastric ceca lumina flanking the distal midgut of H. halys insects and it is acquired each generation when nymphs feed on maternal extrachorion secretions following hatching. Insects prevented from symbiont uptake exhibit developmental delays and aberrant behaviors. To infer contributions of Ca. P. carbekii to H. halys, the complete genome was sequenced and annotated from a North American H. halys population. Overall, the Ca. P. carbekii genome is nearly one-fourth (1.2 Mb) that of free-living congenerics, and retains genes encoding many functions that are potentially host-supportive. Gene content reflects patterns of gene loss/retention typical of intracellular mutualists of plant-feeding insects. Electron and fluorescence in situ microscopic imaging of H. halys egg surfaces revealed that maternal extrachorion secretions were populated with Ca. P. carbekii cells. The reported findings detail a transgenerational mode of symbiont transmission distinct from that observed for intracellular insect mutualists and illustrate the potential additive functions contributed by the bacterial symbiont to this important agricultural pest. Oxford University Press 2015-01-12 /pmc/articles/PMC4350177/ /pubmed/25587021 http://dx.doi.org/10.1093/gbe/evv006 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Kenyon, Laura J. Meulia, Tea Sabree, Zakee L. Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug |
title | Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug |
title_full | Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug |
title_fullStr | Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug |
title_full_unstemmed | Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug |
title_short | Habitat Visualization and Genomic Analysis of “Candidatus Pantoea carbekii,” the Primary Symbiont of the Brown Marmorated Stink Bug |
title_sort | habitat visualization and genomic analysis of “candidatus pantoea carbekii,” the primary symbiont of the brown marmorated stink bug |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350177/ https://www.ncbi.nlm.nih.gov/pubmed/25587021 http://dx.doi.org/10.1093/gbe/evv006 |
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