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Variations in genome-wide RNAi screens: lessons from influenza research

Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed...

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Detalles Bibliográficos
Autores principales: Chou, Yu-Chi, Lai, Michael MC, Wu, Yi-Chen, Hsu, Nai-Chi, Jeng, King-Song, Su, Wen-Chi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4350949/
https://www.ncbi.nlm.nih.gov/pubmed/25745555
http://dx.doi.org/10.1186/s13336-015-0017-5
Descripción
Sumario:Genome-wide RNA interference (RNAi) screening is an emerging and powerful technique for genetic screens, which can be divided into arrayed RNAi screen and pooled RNAi screen/selection based on different screening strategies. To date, several genome-wide RNAi screens have been successfully performed to identify host factors essential for influenza virus replication. However, the host factors identified by different research groups are not always consistent. Taking influenza virus screens as an example, we found that a number of screening parameters may directly or indirectly influence the primary hits identified by the screens. This review highlights the differences among the published genome-wide screening approaches and offers recommendations for performing a good pooled shRNA screen/selection.