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Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves

Leaf tissues of plants usually contain several types of idioblasts, defined as specialized cells whose shape and contents differ from the surrounding homogeneous cells. The spatial patterning of idioblasts, particularly of trichomes and guard cells, across the leaf epidermis has received considerabl...

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Autores principales: Hara, Takuya, Kobayashi, Emi, Ohtsubo, Kohei, Kumada, Shogo, Kanazawa, Mikako, Abe, Tomoko, Itoh, Ryuuichi D., Fujiwara, Makoto T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351012/
https://www.ncbi.nlm.nih.gov/pubmed/25742311
http://dx.doi.org/10.1371/journal.pone.0118965
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author Hara, Takuya
Kobayashi, Emi
Ohtsubo, Kohei
Kumada, Shogo
Kanazawa, Mikako
Abe, Tomoko
Itoh, Ryuuichi D.
Fujiwara, Makoto T.
author_facet Hara, Takuya
Kobayashi, Emi
Ohtsubo, Kohei
Kumada, Shogo
Kanazawa, Mikako
Abe, Tomoko
Itoh, Ryuuichi D.
Fujiwara, Makoto T.
author_sort Hara, Takuya
collection PubMed
description Leaf tissues of plants usually contain several types of idioblasts, defined as specialized cells whose shape and contents differ from the surrounding homogeneous cells. The spatial patterning of idioblasts, particularly of trichomes and guard cells, across the leaf epidermis has received considerable attention as it offers a useful biological model for studying the intercellular regulation of cell fate and patterning. Excretory idioblasts in the leaves of the aquatic monocotyledonous plant Egeria densa produced light blue autofluorescence when irradiated with ultraviolet light. The use of epifluorescence microscopy to detect this autofluorescence provided a simple and convenient method for detecting excretory idioblasts and allowed tracking of those cells across the leaf surfaces, enabling quantitative measurement of the clustering and spacing patterns of idioblasts at the whole leaf level. Occurrence of idioblasts was coordinated along the proximal–distal, medial–lateral, and adaxial–abaxial axes, producing a recognizable consensus spatial pattern of idioblast formation among fully expanded leaves. Idioblast clusters, which comprised up to nine cells aligned along the proximal–distal axis, showed no positional bias or regularity in idioblast-forming areas when compared with singlet idioblasts. Up to 75% of idioblasts existed as clusters on every leaf side examined. The idioblast-forming areas varied between leaves, implying phenotypic plasticity. Furthermore, in young expanding leaves, autofluorescence was occasionally detected in a single giant vesicle or else in one or more small vesicles, which eventually grew to occupy a large portion of the idioblast volume as a central vacuole. Differentiation of vacuoles by accumulating the fluorescence substance might be an integral part of idioblast differentiation. Red autofluorescence from chloroplasts was not detected in idioblasts of young expanding leaves, suggesting idioblast differentiation involves an arrest in chloroplast development at a very early stage, rather than transdifferentiation of chloroplast-containing epidermal cells.
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spelling pubmed-43510122015-03-17 Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves Hara, Takuya Kobayashi, Emi Ohtsubo, Kohei Kumada, Shogo Kanazawa, Mikako Abe, Tomoko Itoh, Ryuuichi D. Fujiwara, Makoto T. PLoS One Research Article Leaf tissues of plants usually contain several types of idioblasts, defined as specialized cells whose shape and contents differ from the surrounding homogeneous cells. The spatial patterning of idioblasts, particularly of trichomes and guard cells, across the leaf epidermis has received considerable attention as it offers a useful biological model for studying the intercellular regulation of cell fate and patterning. Excretory idioblasts in the leaves of the aquatic monocotyledonous plant Egeria densa produced light blue autofluorescence when irradiated with ultraviolet light. The use of epifluorescence microscopy to detect this autofluorescence provided a simple and convenient method for detecting excretory idioblasts and allowed tracking of those cells across the leaf surfaces, enabling quantitative measurement of the clustering and spacing patterns of idioblasts at the whole leaf level. Occurrence of idioblasts was coordinated along the proximal–distal, medial–lateral, and adaxial–abaxial axes, producing a recognizable consensus spatial pattern of idioblast formation among fully expanded leaves. Idioblast clusters, which comprised up to nine cells aligned along the proximal–distal axis, showed no positional bias or regularity in idioblast-forming areas when compared with singlet idioblasts. Up to 75% of idioblasts existed as clusters on every leaf side examined. The idioblast-forming areas varied between leaves, implying phenotypic plasticity. Furthermore, in young expanding leaves, autofluorescence was occasionally detected in a single giant vesicle or else in one or more small vesicles, which eventually grew to occupy a large portion of the idioblast volume as a central vacuole. Differentiation of vacuoles by accumulating the fluorescence substance might be an integral part of idioblast differentiation. Red autofluorescence from chloroplasts was not detected in idioblasts of young expanding leaves, suggesting idioblast differentiation involves an arrest in chloroplast development at a very early stage, rather than transdifferentiation of chloroplast-containing epidermal cells. Public Library of Science 2015-03-05 /pmc/articles/PMC4351012/ /pubmed/25742311 http://dx.doi.org/10.1371/journal.pone.0118965 Text en © 2015 Hara et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hara, Takuya
Kobayashi, Emi
Ohtsubo, Kohei
Kumada, Shogo
Kanazawa, Mikako
Abe, Tomoko
Itoh, Ryuuichi D.
Fujiwara, Makoto T.
Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves
title Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves
title_full Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves
title_fullStr Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves
title_full_unstemmed Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves
title_short Organ-Level Analysis of Idioblast Patterning in Egeria densa Planch. Leaves
title_sort organ-level analysis of idioblast patterning in egeria densa planch. leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351012/
https://www.ncbi.nlm.nih.gov/pubmed/25742311
http://dx.doi.org/10.1371/journal.pone.0118965
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