Comparative Genomics of Cluster O Mycobacteriophages

Mycobacteriophages – viruses of mycobacterial hosts – are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages – Corndog, Catdawg, Dylan, Firecracker, and YungJamal – des...

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Detalles Bibliográficos
Autores principales: Cresawn, Steven G., Pope, Welkin H., Jacobs-Sera, Deborah, Bowman, Charles A., Russell, Daniel A., Dedrick, Rebekah M., Adair, Tamarah, Anders, Kirk R., Ball, Sarah, Bollivar, David, Breitenberger, Caroline, Burnett, Sandra H., Butela, Kristen, Byrnes, Deanna, Carzo, Sarah, Cornely, Kathleen A., Cross, Trevor, Daniels, Richard L., Dunbar, David, Findley, Ann M., Gissendanner, Chris R., Golebiewska, Urszula P., Hartzog, Grant A., Hatherill, J. Robert, Hughes, Lee E., Jalloh, Chernoh S., De Los Santos, Carla, Ekanem, Kevin, Khambule, Sphindile L., King, Rodney A., King-Smith, Christina, Klyczek, Karen, Krukonis, Greg P., Laing, Christian, Lapin, Jonathan S., Lopez, A. Javier, Mkhwanazi, Sipho M., Molloy, Sally D., Moran, Deborah, Munsamy, Vanisha, Pacey, Eddie, Plymale, Ruth, Poxleitner, Marianne, Reyna, Nathan, Schildbach, Joel F., Stukey, Joseph, Taylor, Sarah E., Ware, Vassie C., Wellmann, Amanda L., Westholm, Daniel, Wodarski, Donna, Zajko, Michelle, Zikalala, Thabiso S., Hendrix, Roger W., Hatfull, Graham F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351075/
https://www.ncbi.nlm.nih.gov/pubmed/25742016
http://dx.doi.org/10.1371/journal.pone.0118725
Descripción
Sumario:Mycobacteriophages – viruses of mycobacterial hosts – are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages – Corndog, Catdawg, Dylan, Firecracker, and YungJamal – designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8–9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.

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