Comparative Genomics of Cluster O Mycobacteriophages

Mycobacteriophages – viruses of mycobacterial hosts – are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages – Corndog, Catdawg, Dylan, Firecracker, and YungJamal – des...

Descripción completa

Detalles Bibliográficos
Autores principales: Cresawn, Steven G., Pope, Welkin H., Jacobs-Sera, Deborah, Bowman, Charles A., Russell, Daniel A., Dedrick, Rebekah M., Adair, Tamarah, Anders, Kirk R., Ball, Sarah, Bollivar, David, Breitenberger, Caroline, Burnett, Sandra H., Butela, Kristen, Byrnes, Deanna, Carzo, Sarah, Cornely, Kathleen A., Cross, Trevor, Daniels, Richard L., Dunbar, David, Findley, Ann M., Gissendanner, Chris R., Golebiewska, Urszula P., Hartzog, Grant A., Hatherill, J. Robert, Hughes, Lee E., Jalloh, Chernoh S., De Los Santos, Carla, Ekanem, Kevin, Khambule, Sphindile L., King, Rodney A., King-Smith, Christina, Klyczek, Karen, Krukonis, Greg P., Laing, Christian, Lapin, Jonathan S., Lopez, A. Javier, Mkhwanazi, Sipho M., Molloy, Sally D., Moran, Deborah, Munsamy, Vanisha, Pacey, Eddie, Plymale, Ruth, Poxleitner, Marianne, Reyna, Nathan, Schildbach, Joel F., Stukey, Joseph, Taylor, Sarah E., Ware, Vassie C., Wellmann, Amanda L., Westholm, Daniel, Wodarski, Donna, Zajko, Michelle, Zikalala, Thabiso S., Hendrix, Roger W., Hatfull, Graham F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351075/
https://www.ncbi.nlm.nih.gov/pubmed/25742016
http://dx.doi.org/10.1371/journal.pone.0118725
_version_ 1782360284905603072
author Cresawn, Steven G.
Pope, Welkin H.
Jacobs-Sera, Deborah
Bowman, Charles A.
Russell, Daniel A.
Dedrick, Rebekah M.
Adair, Tamarah
Anders, Kirk R.
Ball, Sarah
Bollivar, David
Breitenberger, Caroline
Burnett, Sandra H.
Butela, Kristen
Byrnes, Deanna
Carzo, Sarah
Cornely, Kathleen A.
Cross, Trevor
Daniels, Richard L.
Dunbar, David
Findley, Ann M.
Gissendanner, Chris R.
Golebiewska, Urszula P.
Hartzog, Grant A.
Hatherill, J. Robert
Hughes, Lee E.
Jalloh, Chernoh S.
De Los Santos, Carla
Ekanem, Kevin
Khambule, Sphindile L.
King, Rodney A.
King-Smith, Christina
Klyczek, Karen
Krukonis, Greg P.
Laing, Christian
Lapin, Jonathan S.
Lopez, A. Javier
Mkhwanazi, Sipho M.
Molloy, Sally D.
Moran, Deborah
Munsamy, Vanisha
Pacey, Eddie
Plymale, Ruth
Poxleitner, Marianne
Reyna, Nathan
Schildbach, Joel F.
Stukey, Joseph
Taylor, Sarah E.
Ware, Vassie C.
Wellmann, Amanda L.
Westholm, Daniel
Wodarski, Donna
Zajko, Michelle
Zikalala, Thabiso S.
Hendrix, Roger W.
Hatfull, Graham F.
author_facet Cresawn, Steven G.
Pope, Welkin H.
Jacobs-Sera, Deborah
Bowman, Charles A.
Russell, Daniel A.
Dedrick, Rebekah M.
Adair, Tamarah
Anders, Kirk R.
Ball, Sarah
Bollivar, David
Breitenberger, Caroline
Burnett, Sandra H.
Butela, Kristen
Byrnes, Deanna
Carzo, Sarah
Cornely, Kathleen A.
Cross, Trevor
Daniels, Richard L.
Dunbar, David
Findley, Ann M.
Gissendanner, Chris R.
Golebiewska, Urszula P.
Hartzog, Grant A.
Hatherill, J. Robert
Hughes, Lee E.
Jalloh, Chernoh S.
De Los Santos, Carla
Ekanem, Kevin
Khambule, Sphindile L.
King, Rodney A.
King-Smith, Christina
Klyczek, Karen
Krukonis, Greg P.
Laing, Christian
Lapin, Jonathan S.
Lopez, A. Javier
Mkhwanazi, Sipho M.
Molloy, Sally D.
Moran, Deborah
Munsamy, Vanisha
Pacey, Eddie
Plymale, Ruth
Poxleitner, Marianne
Reyna, Nathan
Schildbach, Joel F.
Stukey, Joseph
Taylor, Sarah E.
Ware, Vassie C.
Wellmann, Amanda L.
Westholm, Daniel
Wodarski, Donna
Zajko, Michelle
Zikalala, Thabiso S.
Hendrix, Roger W.
Hatfull, Graham F.
author_sort Cresawn, Steven G.
collection PubMed
description Mycobacteriophages – viruses of mycobacterial hosts – are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages – Corndog, Catdawg, Dylan, Firecracker, and YungJamal – designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8–9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange.
format Online
Article
Text
id pubmed-4351075
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-43510752015-03-17 Comparative Genomics of Cluster O Mycobacteriophages Cresawn, Steven G. Pope, Welkin H. Jacobs-Sera, Deborah Bowman, Charles A. Russell, Daniel A. Dedrick, Rebekah M. Adair, Tamarah Anders, Kirk R. Ball, Sarah Bollivar, David Breitenberger, Caroline Burnett, Sandra H. Butela, Kristen Byrnes, Deanna Carzo, Sarah Cornely, Kathleen A. Cross, Trevor Daniels, Richard L. Dunbar, David Findley, Ann M. Gissendanner, Chris R. Golebiewska, Urszula P. Hartzog, Grant A. Hatherill, J. Robert Hughes, Lee E. Jalloh, Chernoh S. De Los Santos, Carla Ekanem, Kevin Khambule, Sphindile L. King, Rodney A. King-Smith, Christina Klyczek, Karen Krukonis, Greg P. Laing, Christian Lapin, Jonathan S. Lopez, A. Javier Mkhwanazi, Sipho M. Molloy, Sally D. Moran, Deborah Munsamy, Vanisha Pacey, Eddie Plymale, Ruth Poxleitner, Marianne Reyna, Nathan Schildbach, Joel F. Stukey, Joseph Taylor, Sarah E. Ware, Vassie C. Wellmann, Amanda L. Westholm, Daniel Wodarski, Donna Zajko, Michelle Zikalala, Thabiso S. Hendrix, Roger W. Hatfull, Graham F. PLoS One Research Article Mycobacteriophages – viruses of mycobacterial hosts – are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages – Corndog, Catdawg, Dylan, Firecracker, and YungJamal – designated as Cluster O with long flexible tails but with unusual prolate capsids. Proteomic analysis of phage Corndog particles, Catdawg particles, and Corndog-infected cells confirms expression of half of the predicted gene products and indicates a non-canonical mechanism for translation of the Corndog tape measure protein. Bioinformatic analysis identifies 8–9 strongly predicted SigA promoters and all five Cluster O genomes contain more than 30 copies of a 17 bp repeat sequence with dyad symmetry located throughout the genomes. Comparison of the Cluster O phages provides insights into phage genome evolution including the processes of gene flux by horizontal genetic exchange. Public Library of Science 2015-03-05 /pmc/articles/PMC4351075/ /pubmed/25742016 http://dx.doi.org/10.1371/journal.pone.0118725 Text en © 2015 Cresawn et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cresawn, Steven G.
Pope, Welkin H.
Jacobs-Sera, Deborah
Bowman, Charles A.
Russell, Daniel A.
Dedrick, Rebekah M.
Adair, Tamarah
Anders, Kirk R.
Ball, Sarah
Bollivar, David
Breitenberger, Caroline
Burnett, Sandra H.
Butela, Kristen
Byrnes, Deanna
Carzo, Sarah
Cornely, Kathleen A.
Cross, Trevor
Daniels, Richard L.
Dunbar, David
Findley, Ann M.
Gissendanner, Chris R.
Golebiewska, Urszula P.
Hartzog, Grant A.
Hatherill, J. Robert
Hughes, Lee E.
Jalloh, Chernoh S.
De Los Santos, Carla
Ekanem, Kevin
Khambule, Sphindile L.
King, Rodney A.
King-Smith, Christina
Klyczek, Karen
Krukonis, Greg P.
Laing, Christian
Lapin, Jonathan S.
Lopez, A. Javier
Mkhwanazi, Sipho M.
Molloy, Sally D.
Moran, Deborah
Munsamy, Vanisha
Pacey, Eddie
Plymale, Ruth
Poxleitner, Marianne
Reyna, Nathan
Schildbach, Joel F.
Stukey, Joseph
Taylor, Sarah E.
Ware, Vassie C.
Wellmann, Amanda L.
Westholm, Daniel
Wodarski, Donna
Zajko, Michelle
Zikalala, Thabiso S.
Hendrix, Roger W.
Hatfull, Graham F.
Comparative Genomics of Cluster O Mycobacteriophages
title Comparative Genomics of Cluster O Mycobacteriophages
title_full Comparative Genomics of Cluster O Mycobacteriophages
title_fullStr Comparative Genomics of Cluster O Mycobacteriophages
title_full_unstemmed Comparative Genomics of Cluster O Mycobacteriophages
title_short Comparative Genomics of Cluster O Mycobacteriophages
title_sort comparative genomics of cluster o mycobacteriophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351075/
https://www.ncbi.nlm.nih.gov/pubmed/25742016
http://dx.doi.org/10.1371/journal.pone.0118725
work_keys_str_mv AT cresawnsteveng comparativegenomicsofclusteromycobacteriophages
AT popewelkinh comparativegenomicsofclusteromycobacteriophages
AT jacobsseradeborah comparativegenomicsofclusteromycobacteriophages
AT bowmancharlesa comparativegenomicsofclusteromycobacteriophages
AT russelldaniela comparativegenomicsofclusteromycobacteriophages
AT dedrickrebekahm comparativegenomicsofclusteromycobacteriophages
AT adairtamarah comparativegenomicsofclusteromycobacteriophages
AT anderskirkr comparativegenomicsofclusteromycobacteriophages
AT ballsarah comparativegenomicsofclusteromycobacteriophages
AT bollivardavid comparativegenomicsofclusteromycobacteriophages
AT breitenbergercaroline comparativegenomicsofclusteromycobacteriophages
AT burnettsandrah comparativegenomicsofclusteromycobacteriophages
AT butelakristen comparativegenomicsofclusteromycobacteriophages
AT byrnesdeanna comparativegenomicsofclusteromycobacteriophages
AT carzosarah comparativegenomicsofclusteromycobacteriophages
AT cornelykathleena comparativegenomicsofclusteromycobacteriophages
AT crosstrevor comparativegenomicsofclusteromycobacteriophages
AT danielsrichardl comparativegenomicsofclusteromycobacteriophages
AT dunbardavid comparativegenomicsofclusteromycobacteriophages
AT findleyannm comparativegenomicsofclusteromycobacteriophages
AT gissendannerchrisr comparativegenomicsofclusteromycobacteriophages
AT golebiewskaurszulap comparativegenomicsofclusteromycobacteriophages
AT hartzoggranta comparativegenomicsofclusteromycobacteriophages
AT hatherilljrobert comparativegenomicsofclusteromycobacteriophages
AT hughesleee comparativegenomicsofclusteromycobacteriophages
AT jallohchernohs comparativegenomicsofclusteromycobacteriophages
AT delossantoscarla comparativegenomicsofclusteromycobacteriophages
AT ekanemkevin comparativegenomicsofclusteromycobacteriophages
AT khambulesphindilel comparativegenomicsofclusteromycobacteriophages
AT kingrodneya comparativegenomicsofclusteromycobacteriophages
AT kingsmithchristina comparativegenomicsofclusteromycobacteriophages
AT klyczekkaren comparativegenomicsofclusteromycobacteriophages
AT krukonisgregp comparativegenomicsofclusteromycobacteriophages
AT laingchristian comparativegenomicsofclusteromycobacteriophages
AT lapinjonathans comparativegenomicsofclusteromycobacteriophages
AT lopezajavier comparativegenomicsofclusteromycobacteriophages
AT mkhwanazisiphom comparativegenomicsofclusteromycobacteriophages
AT molloysallyd comparativegenomicsofclusteromycobacteriophages
AT morandeborah comparativegenomicsofclusteromycobacteriophages
AT munsamyvanisha comparativegenomicsofclusteromycobacteriophages
AT paceyeddie comparativegenomicsofclusteromycobacteriophages
AT plymaleruth comparativegenomicsofclusteromycobacteriophages
AT poxleitnermarianne comparativegenomicsofclusteromycobacteriophages
AT reynanathan comparativegenomicsofclusteromycobacteriophages
AT schildbachjoelf comparativegenomicsofclusteromycobacteriophages
AT stukeyjoseph comparativegenomicsofclusteromycobacteriophages
AT taylorsarahe comparativegenomicsofclusteromycobacteriophages
AT warevassiec comparativegenomicsofclusteromycobacteriophages
AT wellmannamandal comparativegenomicsofclusteromycobacteriophages
AT westholmdaniel comparativegenomicsofclusteromycobacteriophages
AT wodarskidonna comparativegenomicsofclusteromycobacteriophages
AT zajkomichelle comparativegenomicsofclusteromycobacteriophages
AT zikalalathabisos comparativegenomicsofclusteromycobacteriophages
AT hendrixrogerw comparativegenomicsofclusteromycobacteriophages
AT hatfullgrahamf comparativegenomicsofclusteromycobacteriophages

Ejemplares similares