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Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation
OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin suppleme...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351306/ https://www.ncbi.nlm.nih.gov/pubmed/25789524 http://dx.doi.org/10.6061/clinics/2015(02)12 |
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author | Lima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro da Silva Sasso, Gisela Rodrigues Fuchs, Luiz Fernando Portugal de Jesus Simões, Manuel Baracat, Edmund Chada Júnior, José Maria Soares |
author_facet | Lima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro da Silva Sasso, Gisela Rodrigues Fuchs, Luiz Fernando Portugal de Jesus Simões, Manuel Baracat, Edmund Chada Júnior, José Maria Soares |
author_sort | Lima, Gisele Negro |
collection | PubMed |
description | OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip® Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip® Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis. |
format | Online Article Text |
id | pubmed-4351306 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-43513062015-03-16 Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation Lima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro da Silva Sasso, Gisela Rodrigues Fuchs, Luiz Fernando Portugal de Jesus Simões, Manuel Baracat, Edmund Chada Júnior, José Maria Soares Clinics (Sao Paulo) Basic Research OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip® Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip® Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2015-02 /pmc/articles/PMC4351306/ /pubmed/25789524 http://dx.doi.org/10.6061/clinics/2015(02)12 Text en Copyright © 2015 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Basic Research Lima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro da Silva Sasso, Gisela Rodrigues Fuchs, Luiz Fernando Portugal de Jesus Simões, Manuel Baracat, Edmund Chada Júnior, José Maria Soares Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title | Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_full | Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_fullStr | Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_full_unstemmed | Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_short | Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_sort | steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4351306/ https://www.ncbi.nlm.nih.gov/pubmed/25789524 http://dx.doi.org/10.6061/clinics/2015(02)12 |
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