Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China

BACKGROUND: The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital. METHODS: The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. P...

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Autores principales: Zhou, Wanqing, Niu, Dongmei, Cao, Xiaoli, Ning, Mingzhe, Zhang, Zhifeng, Shen, Han, Zhang, Kui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352562/
https://www.ncbi.nlm.nih.gov/pubmed/25888130
http://dx.doi.org/10.1186/s12879-015-0841-z
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author Zhou, Wanqing
Niu, Dongmei
Cao, Xiaoli
Ning, Mingzhe
Zhang, Zhifeng
Shen, Han
Zhang, Kui
author_facet Zhou, Wanqing
Niu, Dongmei
Cao, Xiaoli
Ning, Mingzhe
Zhang, Zhifeng
Shen, Han
Zhang, Kui
author_sort Zhou, Wanqing
collection PubMed
description BACKGROUND: The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital. METHODS: The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE). RESULTS: The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates. CONCLUSIONS: The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.
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spelling pubmed-43525622015-03-09 Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China Zhou, Wanqing Niu, Dongmei Cao, Xiaoli Ning, Mingzhe Zhang, Zhifeng Shen, Han Zhang, Kui BMC Infect Dis Research Article BACKGROUND: The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital. METHODS: The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE). RESULTS: The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates. CONCLUSIONS: The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health. BioMed Central 2015-02-26 /pmc/articles/PMC4352562/ /pubmed/25888130 http://dx.doi.org/10.1186/s12879-015-0841-z Text en © Zhou et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhou, Wanqing
Niu, Dongmei
Cao, Xiaoli
Ning, Mingzhe
Zhang, Zhifeng
Shen, Han
Zhang, Kui
Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China
title Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China
title_full Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China
title_fullStr Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China
title_full_unstemmed Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China
title_short Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China
title_sort clonal dissemination of linezolid-resistant staphylococcus capitis with g2603t mutation in domain v of the 23s rrna and the cfr gene at a tertiary care hospital in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352562/
https://www.ncbi.nlm.nih.gov/pubmed/25888130
http://dx.doi.org/10.1186/s12879-015-0841-z
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