Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells

CRISPR/Cas9 has demonstrated a high-efficiency in site-specific gene targeting. However, potential off-target effects of the Cas9 nuclease represent a major safety concern for any therapeutic application. Here, we knock out the Tafazzin gene by CRISPR/Cas9 in human-induced pluripotent stem cells wit...

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Detalles Bibliográficos
Autores principales: Yang, Luhan, Grishin, Dennis, Wang, Gang, Aach, John, Zhang, Cheng-Zhong, Chari, Raj, Homsy, Jason, Cai, Xuyu, Zhao, Yue, Fan, Jian-Bing, Seidman, Christine, Seidman, Jonathan, Pu, William, Church, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352754/
https://www.ncbi.nlm.nih.gov/pubmed/25425480
http://dx.doi.org/10.1038/ncomms6507

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