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It takes acid, rather than ice, to freeze glucose

Plasma glucose levels provide the cornerstone of diabetes evaluation. Unfortunately, glucose levels drop in vitro due to glycolysis. Guidelines provide suitable conditions which minimize glycolysis, such as immediate centrifugation or the use of ice/water slurry storage containers. For obvious pract...

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Autores principales: van den Berg, S. A. A., Thelen, M. H. M., Salden, L. P. W., van Thiel, S. W., Boonen, K. J. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352852/
https://www.ncbi.nlm.nih.gov/pubmed/25748167
http://dx.doi.org/10.1038/srep08875
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author van den Berg, S. A. A.
Thelen, M. H. M.
Salden, L. P. W.
van Thiel, S. W.
Boonen, K. J. M.
author_facet van den Berg, S. A. A.
Thelen, M. H. M.
Salden, L. P. W.
van Thiel, S. W.
Boonen, K. J. M.
author_sort van den Berg, S. A. A.
collection PubMed
description Plasma glucose levels provide the cornerstone of diabetes evaluation. Unfortunately, glucose levels drop in vitro due to glycolysis. Guidelines provide suitable conditions which minimize glycolysis, such as immediate centrifugation or the use of ice/water slurry storage containers. For obvious practical reasons, most laboratories use blood collection tubes containing glycolysis inhibitors. We describe the effect of a variety of commonly used blood collection tubes on in vitro stability of glucose. Furthermore, we looked at the validity of the assumption that glycolytic activity is minimal when blood is kept in an ice/water slurry. Sodium fluoride alone does not reduce in vitro glycolysis in the first 120 minutes after phlebotomy. Addition of citrate almost completely prevented in vitro glycolysis, but showed a positive bias (0.2 mmol/l) compared to control. This is partly due to a small drop in glucose level in control blood, drawn according to the current guidelines. This drop occurs within 15 minutes, in which glycolysis has been described to be minimal and acceptable. NaF-EDTA-citrate based test tubes provide the best pre-analytical condition available. Furthermore, glucose levels are not stable in heparinized blood placed in an ice/water slurry. We strongly advise the use of NaF-EDTA-citrate based test tubes in diabetes research.
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spelling pubmed-43528522015-03-17 It takes acid, rather than ice, to freeze glucose van den Berg, S. A. A. Thelen, M. H. M. Salden, L. P. W. van Thiel, S. W. Boonen, K. J. M. Sci Rep Article Plasma glucose levels provide the cornerstone of diabetes evaluation. Unfortunately, glucose levels drop in vitro due to glycolysis. Guidelines provide suitable conditions which minimize glycolysis, such as immediate centrifugation or the use of ice/water slurry storage containers. For obvious practical reasons, most laboratories use blood collection tubes containing glycolysis inhibitors. We describe the effect of a variety of commonly used blood collection tubes on in vitro stability of glucose. Furthermore, we looked at the validity of the assumption that glycolytic activity is minimal when blood is kept in an ice/water slurry. Sodium fluoride alone does not reduce in vitro glycolysis in the first 120 minutes after phlebotomy. Addition of citrate almost completely prevented in vitro glycolysis, but showed a positive bias (0.2 mmol/l) compared to control. This is partly due to a small drop in glucose level in control blood, drawn according to the current guidelines. This drop occurs within 15 minutes, in which glycolysis has been described to be minimal and acceptable. NaF-EDTA-citrate based test tubes provide the best pre-analytical condition available. Furthermore, glucose levels are not stable in heparinized blood placed in an ice/water slurry. We strongly advise the use of NaF-EDTA-citrate based test tubes in diabetes research. Nature Publishing Group 2015-03-09 /pmc/articles/PMC4352852/ /pubmed/25748167 http://dx.doi.org/10.1038/srep08875 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
van den Berg, S. A. A.
Thelen, M. H. M.
Salden, L. P. W.
van Thiel, S. W.
Boonen, K. J. M.
It takes acid, rather than ice, to freeze glucose
title It takes acid, rather than ice, to freeze glucose
title_full It takes acid, rather than ice, to freeze glucose
title_fullStr It takes acid, rather than ice, to freeze glucose
title_full_unstemmed It takes acid, rather than ice, to freeze glucose
title_short It takes acid, rather than ice, to freeze glucose
title_sort it takes acid, rather than ice, to freeze glucose
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352852/
https://www.ncbi.nlm.nih.gov/pubmed/25748167
http://dx.doi.org/10.1038/srep08875
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