A reproducible method for the expansion of mouse CD8 + T lymphocytes

Murine adoptive CD8 + T-cell immunotherapy studies require the generation of large numbers of high viability CD8 + cells. Here we report a tissue culture protocol for the reliable expansion of CD8 + T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells wer...

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Detalles Bibliográficos
Autores principales: Lewis, Mark D., de Leenheer, Evy, Fishman, Sigal, Siew, Lai Khai, Gross, Gideon, Wong, F. Susan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352898/
https://www.ncbi.nlm.nih.gov/pubmed/25602136
http://dx.doi.org/10.1016/j.jim.2015.01.004
Descripción
Sumario:Murine adoptive CD8 + T-cell immunotherapy studies require the generation of large numbers of high viability CD8 + cells. Here we report a tissue culture protocol for the reliable expansion of CD8 + T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week.

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