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A reproducible method for the expansion of mouse CD8 + T lymphocytes

Murine adoptive CD8 + T-cell immunotherapy studies require the generation of large numbers of high viability CD8 + cells. Here we report a tissue culture protocol for the reliable expansion of CD8 + T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells wer...

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Detalles Bibliográficos
Autores principales:	Lewis, Mark D., de Leenheer, Evy, Fishman, Sigal, Siew, Lai Khai, Gross, Gideon, Wong, F. Susan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2015
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4352898/ https://www.ncbi.nlm.nih.gov/pubmed/25602136 http://dx.doi.org/10.1016/j.jim.2015.01.004

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