Reversible Energy-Transfer Switching on a DNA Scaffold

[Image: see text] We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV...

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Detalles Bibliográficos
Autores principales: Bälter, Magnus, Hammarson, Martin, Remón, Patricia, Li, Shiming, Gale, Nittaya, Brown, Tom, Andréasson, Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353014/
https://www.ncbi.nlm.nih.gov/pubmed/25687828
http://dx.doi.org/10.1021/ja512416n
Descripción
Sumario:[Image: see text] We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

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