Reversible Energy-Transfer Switching on a DNA Scaffold

[Image: see text] We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV...

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Autores principales: Bälter, Magnus, Hammarson, Martin, Remón, Patricia, Li, Shiming, Gale, Nittaya, Brown, Tom, Andréasson, Joakim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353014/
https://www.ncbi.nlm.nih.gov/pubmed/25687828
http://dx.doi.org/10.1021/ja512416n
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author Bälter, Magnus
Hammarson, Martin
Remón, Patricia
Li, Shiming
Gale, Nittaya
Brown, Tom
Andréasson, Joakim
author_facet Bälter, Magnus
Hammarson, Martin
Remón, Patricia
Li, Shiming
Gale, Nittaya
Brown, Tom
Andréasson, Joakim
author_sort Bälter, Magnus
collection PubMed
description [Image: see text] We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.
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spelling pubmed-43530142015-03-11 Reversible Energy-Transfer Switching on a DNA Scaffold Bälter, Magnus Hammarson, Martin Remón, Patricia Li, Shiming Gale, Nittaya Brown, Tom Andréasson, Joakim J Am Chem Soc [Image: see text] We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer. American Chemical Society 2015-02-17 2015-02-25 /pmc/articles/PMC4353014/ /pubmed/25687828 http://dx.doi.org/10.1021/ja512416n Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Bälter, Magnus
Hammarson, Martin
Remón, Patricia
Li, Shiming
Gale, Nittaya
Brown, Tom
Andréasson, Joakim
Reversible Energy-Transfer Switching on a DNA Scaffold
title Reversible Energy-Transfer Switching on a DNA Scaffold
title_full Reversible Energy-Transfer Switching on a DNA Scaffold
title_fullStr Reversible Energy-Transfer Switching on a DNA Scaffold
title_full_unstemmed Reversible Energy-Transfer Switching on a DNA Scaffold
title_short Reversible Energy-Transfer Switching on a DNA Scaffold
title_sort reversible energy-transfer switching on a dna scaffold
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353014/
https://www.ncbi.nlm.nih.gov/pubmed/25687828
http://dx.doi.org/10.1021/ja512416n
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