Cargando…

Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis

BACKGROUND: Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES: This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rh...

Descripción completa

Detalles Bibliográficos
Autores principales: Zahiri Yeganeh, Samaneh, Ataee, Ramezan Ali, Alishiri, Gholam Hossein, Movahedi, Monireh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353035/
https://www.ncbi.nlm.nih.gov/pubmed/25793096
http://dx.doi.org/10.5812/jjm.16621
_version_ 1782360545404387328
author Zahiri Yeganeh, Samaneh
Ataee, Ramezan Ali
Alishiri, Gholam Hossein
Movahedi, Monireh
author_facet Zahiri Yeganeh, Samaneh
Ataee, Ramezan Ali
Alishiri, Gholam Hossein
Movahedi, Monireh
author_sort Zahiri Yeganeh, Samaneh
collection PubMed
description BACKGROUND: Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES: This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rheumatoid arthritis. PATIENTS AND METHODS: A total of 83 blood samples of patients with RA were studied. All of patient’s blood samples have been cultured. Polymerase chain reaction (PCR) and ELISA methods have been used to assess the existence of staphylococcal enterotoxin E (entE). The data were analyzed through descriptive statistics. RESULTS: During this study and after sequential sub cultures, only 5 bacterial strains were isolated. Based on the results of biochemical tests, just one case was detected as Staphylococcus aureus. The result of molecular diagnosis of enterotoxin E gene was 13.25%. The results of ELISA were 40.96% positive for staphylococcal enterotoxin E. CONCLUSIONS: In this study, staphylococcal enterotoxin E (superantigen E) was detected in the blood of patients with RA, but its origin is unknown, because no staphylococcus enterotoxin E producer was isolated. This finding could provide a good model for the diagnosis and treatment of RA. However, the results of this study have shown some evidence regarding endogenous origin of involved superantigens in patients with rheumatoid arthritis.
format Online
Article
Text
id pubmed-4353035
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Kowsar
record_format MEDLINE/PubMed
spelling pubmed-43530352015-03-19 Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis Zahiri Yeganeh, Samaneh Ataee, Ramezan Ali Alishiri, Gholam Hossein Movahedi, Monireh Jundishapur J Microbiol Research Article BACKGROUND: Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES: This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rheumatoid arthritis. PATIENTS AND METHODS: A total of 83 blood samples of patients with RA were studied. All of patient’s blood samples have been cultured. Polymerase chain reaction (PCR) and ELISA methods have been used to assess the existence of staphylococcal enterotoxin E (entE). The data were analyzed through descriptive statistics. RESULTS: During this study and after sequential sub cultures, only 5 bacterial strains were isolated. Based on the results of biochemical tests, just one case was detected as Staphylococcus aureus. The result of molecular diagnosis of enterotoxin E gene was 13.25%. The results of ELISA were 40.96% positive for staphylococcal enterotoxin E. CONCLUSIONS: In this study, staphylococcal enterotoxin E (superantigen E) was detected in the blood of patients with RA, but its origin is unknown, because no staphylococcus enterotoxin E producer was isolated. This finding could provide a good model for the diagnosis and treatment of RA. However, the results of this study have shown some evidence regarding endogenous origin of involved superantigens in patients with rheumatoid arthritis. Kowsar 2015-02-18 /pmc/articles/PMC4353035/ /pubmed/25793096 http://dx.doi.org/10.5812/jjm.16621 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Zahiri Yeganeh, Samaneh
Ataee, Ramezan Ali
Alishiri, Gholam Hossein
Movahedi, Monireh
Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
title Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
title_full Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
title_fullStr Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
title_full_unstemmed Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
title_short Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
title_sort bacteriological and molecular assessment of staphylococcal enterotoxin e in the blood of patients with rheumatoid arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353035/
https://www.ncbi.nlm.nih.gov/pubmed/25793096
http://dx.doi.org/10.5812/jjm.16621
work_keys_str_mv AT zahiriyeganehsamaneh bacteriologicalandmolecularassessmentofstaphylococcalenterotoxineinthebloodofpatientswithrheumatoidarthritis
AT ataeeramezanali bacteriologicalandmolecularassessmentofstaphylococcalenterotoxineinthebloodofpatientswithrheumatoidarthritis
AT alishirigholamhossein bacteriologicalandmolecularassessmentofstaphylococcalenterotoxineinthebloodofpatientswithrheumatoidarthritis
AT movahedimonireh bacteriologicalandmolecularassessmentofstaphylococcalenterotoxineinthebloodofpatientswithrheumatoidarthritis