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Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis
BACKGROUND: Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES: This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rh...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353035/ https://www.ncbi.nlm.nih.gov/pubmed/25793096 http://dx.doi.org/10.5812/jjm.16621 |
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author | Zahiri Yeganeh, Samaneh Ataee, Ramezan Ali Alishiri, Gholam Hossein Movahedi, Monireh |
author_facet | Zahiri Yeganeh, Samaneh Ataee, Ramezan Ali Alishiri, Gholam Hossein Movahedi, Monireh |
author_sort | Zahiri Yeganeh, Samaneh |
collection | PubMed |
description | BACKGROUND: Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES: This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rheumatoid arthritis. PATIENTS AND METHODS: A total of 83 blood samples of patients with RA were studied. All of patient’s blood samples have been cultured. Polymerase chain reaction (PCR) and ELISA methods have been used to assess the existence of staphylococcal enterotoxin E (entE). The data were analyzed through descriptive statistics. RESULTS: During this study and after sequential sub cultures, only 5 bacterial strains were isolated. Based on the results of biochemical tests, just one case was detected as Staphylococcus aureus. The result of molecular diagnosis of enterotoxin E gene was 13.25%. The results of ELISA were 40.96% positive for staphylococcal enterotoxin E. CONCLUSIONS: In this study, staphylococcal enterotoxin E (superantigen E) was detected in the blood of patients with RA, but its origin is unknown, because no staphylococcus enterotoxin E producer was isolated. This finding could provide a good model for the diagnosis and treatment of RA. However, the results of this study have shown some evidence regarding endogenous origin of involved superantigens in patients with rheumatoid arthritis. |
format | Online Article Text |
id | pubmed-4353035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-43530352015-03-19 Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis Zahiri Yeganeh, Samaneh Ataee, Ramezan Ali Alishiri, Gholam Hossein Movahedi, Monireh Jundishapur J Microbiol Research Article BACKGROUND: Rheumatoid arthritis (RA) is the most common chronic inflammatory disease of unknown etiology. In this regard, the role of bacterial superantigens (as an effective agent) were considered. OBJECTIVES: This study aimed to assess staphylococcal enterotoxin E in the blood of patients with rheumatoid arthritis. PATIENTS AND METHODS: A total of 83 blood samples of patients with RA were studied. All of patient’s blood samples have been cultured. Polymerase chain reaction (PCR) and ELISA methods have been used to assess the existence of staphylococcal enterotoxin E (entE). The data were analyzed through descriptive statistics. RESULTS: During this study and after sequential sub cultures, only 5 bacterial strains were isolated. Based on the results of biochemical tests, just one case was detected as Staphylococcus aureus. The result of molecular diagnosis of enterotoxin E gene was 13.25%. The results of ELISA were 40.96% positive for staphylococcal enterotoxin E. CONCLUSIONS: In this study, staphylococcal enterotoxin E (superantigen E) was detected in the blood of patients with RA, but its origin is unknown, because no staphylococcus enterotoxin E producer was isolated. This finding could provide a good model for the diagnosis and treatment of RA. However, the results of this study have shown some evidence regarding endogenous origin of involved superantigens in patients with rheumatoid arthritis. Kowsar 2015-02-18 /pmc/articles/PMC4353035/ /pubmed/25793096 http://dx.doi.org/10.5812/jjm.16621 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Zahiri Yeganeh, Samaneh Ataee, Ramezan Ali Alishiri, Gholam Hossein Movahedi, Monireh Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis |
title | Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis |
title_full | Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis |
title_fullStr | Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis |
title_full_unstemmed | Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis |
title_short | Bacteriological and Molecular Assessment of Staphylococcal Enterotoxin E in the Blood of Patients With Rheumatoid Arthritis |
title_sort | bacteriological and molecular assessment of staphylococcal enterotoxin e in the blood of patients with rheumatoid arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353035/ https://www.ncbi.nlm.nih.gov/pubmed/25793096 http://dx.doi.org/10.5812/jjm.16621 |
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