Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆

In the present study, we transplanted bone marrow-derived mesenchymal stem cells into the CA3 area of the hippocampus of chronic epilepsy rats kindled by lithium chloride-pilocarpine. Immunofluorescence and western blotting revealed an increase in adenosine A1 receptor expression and a decrease in a...

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Autores principales: Kang, Huicong, Hu, Qi, Liu, Xiaoyan, Liu, Yinhe, Xu, Feng, Li, Xiang, Zhu, Suiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report: Stem Cells and Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353095/
https://www.ncbi.nlm.nih.gov/pubmed/25806064
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.002
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author Kang, Huicong
Hu, Qi
Liu, Xiaoyan
Liu, Yinhe
Xu, Feng
Li, Xiang
Zhu, Suiqiang
author_facet Kang, Huicong
Hu, Qi
Liu, Xiaoyan
Liu, Yinhe
Xu, Feng
Li, Xiang
Zhu, Suiqiang
author_sort Kang, Huicong
collection PubMed
description In the present study, we transplanted bone marrow-derived mesenchymal stem cells into the CA3 area of the hippocampus of chronic epilepsy rats kindled by lithium chloride-pilocarpine. Immunofluorescence and western blotting revealed an increase in adenosine A1 receptor expression and a decrease in adenosine A2a receptor expression in the brain tissues of epileptic rats 3 months after transplantation. Moreover, the imbalance in the A1 adenosine receptor/A2a adenosine receptor ratio was improved. Electroencephalograms showed that frequency and amplitude of spikes in the hippocampus and frontal lobe were reduced. These results suggested that mesenchymal stem cell transplantation can reconstruct the normal function of the adenosine system in the brain and greatly improve epileptiform discharges.
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spelling pubmed-43530952015-03-24 Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆ Kang, Huicong Hu, Qi Liu, Xiaoyan Liu, Yinhe Xu, Feng Li, Xiang Zhu, Suiqiang Neural Regen Res Research and Report: Stem Cells and Neural Regeneration In the present study, we transplanted bone marrow-derived mesenchymal stem cells into the CA3 area of the hippocampus of chronic epilepsy rats kindled by lithium chloride-pilocarpine. Immunofluorescence and western blotting revealed an increase in adenosine A1 receptor expression and a decrease in adenosine A2a receptor expression in the brain tissues of epileptic rats 3 months after transplantation. Moreover, the imbalance in the A1 adenosine receptor/A2a adenosine receptor ratio was improved. Electroencephalograms showed that frequency and amplitude of spikes in the hippocampus and frontal lobe were reduced. These results suggested that mesenchymal stem cell transplantation can reconstruct the normal function of the adenosine system in the brain and greatly improve epileptiform discharges. Medknow Publications & Media Pvt Ltd 2012-02-05 /pmc/articles/PMC4353095/ /pubmed/25806064 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.002 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Stem Cells and Neural Regeneration
Kang, Huicong
Hu, Qi
Liu, Xiaoyan
Liu, Yinhe
Xu, Feng
Li, Xiang
Zhu, Suiqiang
Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
title Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
title_full Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
title_fullStr Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
title_full_unstemmed Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
title_short Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
title_sort reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation☆
topic Research and Report: Stem Cells and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353095/
https://www.ncbi.nlm.nih.gov/pubmed/25806064
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.002
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