The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆

The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction (active principle region of de...

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Autores principales: Zheng, Jinghui, Wan, Yi, Chi, Jianhuai, Shen, Dekai, Wu, Tingting, Li, Weimin, Du, Pengcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Research and Report: Stem Cells and Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353097/
https://www.ncbi.nlm.nih.gov/pubmed/25806066
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.004
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author Zheng, Jinghui
Wan, Yi
Chi, Jianhuai
Shen, Dekai
Wu, Tingting
Li, Weimin
Du, Pengcheng
author_facet Zheng, Jinghui
Wan, Yi
Chi, Jianhuai
Shen, Dekai
Wu, Tingting
Li, Weimin
Du, Pengcheng
author_sort Zheng, Jinghui
collection PubMed
description The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction (active principle region of decoction for invigorating yang for recuperation). After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula.
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spelling pubmed-43530972015-03-24 The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆ Zheng, Jinghui Wan, Yi Chi, Jianhuai Shen, Dekai Wu, Tingting Li, Weimin Du, Pengcheng Neural Regen Res Research and Report: Stem Cells and Neural Regeneration The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction (active principle region of decoction for invigorating yang for recuperation). After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula. Medknow Publications & Media Pvt Ltd 2012-02-05 /pmc/articles/PMC4353097/ /pubmed/25806066 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.004 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report: Stem Cells and Neural Regeneration
Zheng, Jinghui
Wan, Yi
Chi, Jianhuai
Shen, Dekai
Wu, Tingting
Li, Weimin
Du, Pengcheng
The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆
title The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆
title_full The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆
title_fullStr The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆
title_full_unstemmed The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆
title_short The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: Superior effects over original formula of Buyang Huanwu decoction☆
title_sort active principle region of buyang huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells: superior effects over original formula of buyang huanwu decoction☆
topic Research and Report: Stem Cells and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353097/
https://www.ncbi.nlm.nih.gov/pubmed/25806066
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.004
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