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Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆

In this study, we established a rat model of optic nerve crush to explore the effects of erythropoietin on retinal ganglion cell axonal regeneration. At 15 days after injury in erythropoietin treated rats, retinal ganglion cell densities in regions corresponding to the 1/6, 3/6 and 5/6 ratios of the...

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Autores principales: Tan, Haibo, Kang, Xin, Zhong, Yisheng, Shen, Xi, Cheng, Yu, Jiao, Qin, Deng, Lianfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353103/
https://www.ncbi.nlm.nih.gov/pubmed/25806072
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.010
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author Tan, Haibo
Kang, Xin
Zhong, Yisheng
Shen, Xi
Cheng, Yu
Jiao, Qin
Deng, Lianfu
author_facet Tan, Haibo
Kang, Xin
Zhong, Yisheng
Shen, Xi
Cheng, Yu
Jiao, Qin
Deng, Lianfu
author_sort Tan, Haibo
collection PubMed
description In this study, we established a rat model of optic nerve crush to explore the effects of erythropoietin on retinal ganglion cell axonal regeneration. At 15 days after injury in erythropoietin treated rats, retinal ganglion cell densities in regions corresponding to the 1/6, 3/6 and 5/6 ratios of the retinal radius were significantly increased. In addition, the number of growth associated protein-43 positive axons was significantly increased at different distances (50, 250 and 500 μm) from the crush site after erythropoietin treatment. Erythropoietin significantly increased growth associated protein-43 protein levels in the retina after crush injury, as determined by western blot and immunofluorescence analysis. These results demonstrate that erythropoietin protects injured retinal ganglion cells and promotes axonal regeneration.
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spelling pubmed-43531032015-03-24 Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆ Tan, Haibo Kang, Xin Zhong, Yisheng Shen, Xi Cheng, Yu Jiao, Qin Deng, Lianfu Neural Regen Res Technique and Method: Nerve Factor and Neuroregeneration In this study, we established a rat model of optic nerve crush to explore the effects of erythropoietin on retinal ganglion cell axonal regeneration. At 15 days after injury in erythropoietin treated rats, retinal ganglion cell densities in regions corresponding to the 1/6, 3/6 and 5/6 ratios of the retinal radius were significantly increased. In addition, the number of growth associated protein-43 positive axons was significantly increased at different distances (50, 250 and 500 μm) from the crush site after erythropoietin treatment. Erythropoietin significantly increased growth associated protein-43 protein levels in the retina after crush injury, as determined by western blot and immunofluorescence analysis. These results demonstrate that erythropoietin protects injured retinal ganglion cells and promotes axonal regeneration. Medknow Publications & Media Pvt Ltd 2012-02-05 /pmc/articles/PMC4353103/ /pubmed/25806072 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.010 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technique and Method: Nerve Factor and Neuroregeneration
Tan, Haibo
Kang, Xin
Zhong, Yisheng
Shen, Xi
Cheng, Yu
Jiao, Qin
Deng, Lianfu
Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
title Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
title_full Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
title_fullStr Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
title_full_unstemmed Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
title_short Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
title_sort erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush☆
topic Technique and Method: Nerve Factor and Neuroregeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353103/
https://www.ncbi.nlm.nih.gov/pubmed/25806072
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.04.010
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