Identification of structural DNA variations in human cell cultures after long-term passage

Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells dem...

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Autores principales: Pavlova, GV, Vergun, AA, Rybalkina, EY, Butovskaya, PR, Ryskov, AP
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353222/
https://www.ncbi.nlm.nih.gov/pubmed/25607645
http://dx.doi.org/10.4161/15384101.2014.974427
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author Pavlova, GV
Vergun, AA
Rybalkina, EY
Butovskaya, PR
Ryskov, AP
author_facet Pavlova, GV
Vergun, AA
Rybalkina, EY
Butovskaya, PR
Ryskov, AP
author_sort Pavlova, GV
collection PubMed
description Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells demonstrated increased DNA instability. RAPD fragments from different cell lines after different number of passages were cloned and sequenced. The chromosomal localization of these fragments was identified and single-nucleotide variations in RAPD fragments isolated from cell lines after 8–12 passages were revealed. Some of them had permanent localization, while most variations demonstrated random distribution and can be considered as de novo mutations.
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spelling pubmed-43532222016-01-17 Identification of structural DNA variations in human cell cultures after long-term passage Pavlova, GV Vergun, AA Rybalkina, EY Butovskaya, PR Ryskov, AP Cell Cycle Reports Random amplified polymorphic DNA (RAPD) analysis was adapted for genomic identification of cell cultures and evaluation of DNA stability in cells of different origin at different culture passages. DNA stability was observed in cultures after no more than 5 passages. Adipose-derived stromal cells demonstrated increased DNA instability. RAPD fragments from different cell lines after different number of passages were cloned and sequenced. The chromosomal localization of these fragments was identified and single-nucleotide variations in RAPD fragments isolated from cell lines after 8–12 passages were revealed. Some of them had permanent localization, while most variations demonstrated random distribution and can be considered as de novo mutations. Taylor & Francis 2015-01-17 /pmc/articles/PMC4353222/ /pubmed/25607645 http://dx.doi.org/10.4161/15384101.2014.974427 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Reports
Pavlova, GV
Vergun, AA
Rybalkina, EY
Butovskaya, PR
Ryskov, AP
Identification of structural DNA variations in human cell cultures after long-term passage
title Identification of structural DNA variations in human cell cultures after long-term passage
title_full Identification of structural DNA variations in human cell cultures after long-term passage
title_fullStr Identification of structural DNA variations in human cell cultures after long-term passage
title_full_unstemmed Identification of structural DNA variations in human cell cultures after long-term passage
title_short Identification of structural DNA variations in human cell cultures after long-term passage
title_sort identification of structural dna variations in human cell cultures after long-term passage
topic Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353222/
https://www.ncbi.nlm.nih.gov/pubmed/25607645
http://dx.doi.org/10.4161/15384101.2014.974427
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