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Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage

A key objective in microbial biofuels strain development is to maximize carbon flux to target products while minimizing cell biomass accumulation, such that ideally the algae and bacteria would operate in a photo-catalytic state. A brief period of such a physiological state has recently been demonst...

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Autores principales: Carrieri, Damian, Broadbent, Charlie, Carruth, David, Paddock, Troy, Ungerer, Justin, Maness, Pin-Ching, Ghirardi, Maria, Yu, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353341/
https://www.ncbi.nlm.nih.gov/pubmed/25616027
http://dx.doi.org/10.1111/1751-7915.12243
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author Carrieri, Damian
Broadbent, Charlie
Carruth, David
Paddock, Troy
Ungerer, Justin
Maness, Pin-Ching
Ghirardi, Maria
Yu, Jianping
author_facet Carrieri, Damian
Broadbent, Charlie
Carruth, David
Paddock, Troy
Ungerer, Justin
Maness, Pin-Ching
Ghirardi, Maria
Yu, Jianping
author_sort Carrieri, Damian
collection PubMed
description A key objective in microbial biofuels strain development is to maximize carbon flux to target products while minimizing cell biomass accumulation, such that ideally the algae and bacteria would operate in a photo-catalytic state. A brief period of such a physiological state has recently been demonstrated in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC strain incapable of glycogen storage. When deprived of nitrogen, the ΔglgC excretes the organic acids alpha-ketoglutarate and pyruvate for a number of days without increasing cell biomass. This study examines the relationship between the growth state and the photo-catalytic state, and characterizes the metabolic adaptability of the photo-catalytic state to increasing light intensity. It is found that the culture can transition naturally from the growth state into the photo-catalytic state when provided with limited nitrogen supply during the growth phase. Photosynthetic capacity and pigments are lost over time in the photo-catalytic state. Reversal to growth state is observed with re-addition of nitrogen nutrient, accompanied by restoration of photosynthetic capacity and pigment levels in the cells. While the overall productivity increased under high light conditions, the ratio of alpha-ketoglutarate/pyruvate is altered, suggesting that carbon partition between the two products is adaptable to environmental conditions.
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spelling pubmed-43533412015-03-12 Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage Carrieri, Damian Broadbent, Charlie Carruth, David Paddock, Troy Ungerer, Justin Maness, Pin-Ching Ghirardi, Maria Yu, Jianping Microb Biotechnol Research Articles A key objective in microbial biofuels strain development is to maximize carbon flux to target products while minimizing cell biomass accumulation, such that ideally the algae and bacteria would operate in a photo-catalytic state. A brief period of such a physiological state has recently been demonstrated in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC strain incapable of glycogen storage. When deprived of nitrogen, the ΔglgC excretes the organic acids alpha-ketoglutarate and pyruvate for a number of days without increasing cell biomass. This study examines the relationship between the growth state and the photo-catalytic state, and characterizes the metabolic adaptability of the photo-catalytic state to increasing light intensity. It is found that the culture can transition naturally from the growth state into the photo-catalytic state when provided with limited nitrogen supply during the growth phase. Photosynthetic capacity and pigments are lost over time in the photo-catalytic state. Reversal to growth state is observed with re-addition of nitrogen nutrient, accompanied by restoration of photosynthetic capacity and pigment levels in the cells. While the overall productivity increased under high light conditions, the ratio of alpha-ketoglutarate/pyruvate is altered, suggesting that carbon partition between the two products is adaptable to environmental conditions. BlackWell Publishing Ltd 2015-03 2015-01-23 /pmc/articles/PMC4353341/ /pubmed/25616027 http://dx.doi.org/10.1111/1751-7915.12243 Text en Journal compilation © 2015 John Wiley & Sons Ltd and Society for Applied Microbiology http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Carrieri, Damian
Broadbent, Charlie
Carruth, David
Paddock, Troy
Ungerer, Justin
Maness, Pin-Ching
Ghirardi, Maria
Yu, Jianping
Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage
title Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage
title_full Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage
title_fullStr Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage
title_full_unstemmed Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage
title_short Enhancing photo-catalytic production of organic acids in the cyanobacterium Synechocystis sp. PCC 6803 ΔglgC, a strain incapable of glycogen storage
title_sort enhancing photo-catalytic production of organic acids in the cyanobacterium synechocystis sp. pcc 6803 δglgc, a strain incapable of glycogen storage
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353341/
https://www.ncbi.nlm.nih.gov/pubmed/25616027
http://dx.doi.org/10.1111/1751-7915.12243
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