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Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer

Aim. We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC). Patients and Methods. 89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool sam...

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Autores principales: Li, Wen-han, Zhang, Hao, Guo, Qi, Wu, Xuan-di, Xu, Zi-sen, Dang, Cheng-xue, Xia, Peng, Song, Yong-chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353443/
https://www.ncbi.nlm.nih.gov/pubmed/25802477
http://dx.doi.org/10.1155/2015/657570
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author Li, Wen-han
Zhang, Hao
Guo, Qi
Wu, Xuan-di
Xu, Zi-sen
Dang, Cheng-xue
Xia, Peng
Song, Yong-chun
author_facet Li, Wen-han
Zhang, Hao
Guo, Qi
Wu, Xuan-di
Xu, Zi-sen
Dang, Cheng-xue
Xia, Peng
Song, Yong-chun
author_sort Li, Wen-han
collection PubMed
description Aim. We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC). Patients and Methods. 89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool samples were collected as healthy controls. Results. The methylation level of those samples was measured by methylation-specific polymerase chain reaction (MSP). The result shows that compared with the paired controls, both SNCA and FBN1 were significantly hypermethylated in CRC patients in tissue samples (P < 0.001). In the stool samples, hypermethylated SNCA and FBN1 were detected to be significantly higher than that in normal stool samples (P < 0.001). The combined sensitivity of at least one positive among the two markers in stool samples was 84.3%, with a specificity of 93.3%. In addition, our experiment suggested that the positive rates of SNCA and FBN1 in Dukes A stage were significantly higher than that of FOBT (P = 0.039; P = 0.006, resp.). Conclusion. We concluded that methylation testing of SNCA and FBN1 genes in stool sample may offer a good alternative in a simple, promising, and noninvasive detection of colorectal cancer.
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spelling pubmed-43534432015-03-23 Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer Li, Wen-han Zhang, Hao Guo, Qi Wu, Xuan-di Xu, Zi-sen Dang, Cheng-xue Xia, Peng Song, Yong-chun Dis Markers Research Article Aim. We examined the methylation status of SNCA and FBN1 genes in patients' paired tissue and stool samples for detection of colorectal cancer (CRC). Patients and Methods. 89 DNA tissue samples (normal/cancer) and corresponding stool samples were analyzed in our study. In addition, 30 stool samples were collected as healthy controls. Results. The methylation level of those samples was measured by methylation-specific polymerase chain reaction (MSP). The result shows that compared with the paired controls, both SNCA and FBN1 were significantly hypermethylated in CRC patients in tissue samples (P < 0.001). In the stool samples, hypermethylated SNCA and FBN1 were detected to be significantly higher than that in normal stool samples (P < 0.001). The combined sensitivity of at least one positive among the two markers in stool samples was 84.3%, with a specificity of 93.3%. In addition, our experiment suggested that the positive rates of SNCA and FBN1 in Dukes A stage were significantly higher than that of FOBT (P = 0.039; P = 0.006, resp.). Conclusion. We concluded that methylation testing of SNCA and FBN1 genes in stool sample may offer a good alternative in a simple, promising, and noninvasive detection of colorectal cancer. Hindawi Publishing Corporation 2015 2015-02-23 /pmc/articles/PMC4353443/ /pubmed/25802477 http://dx.doi.org/10.1155/2015/657570 Text en Copyright © 2015 Wen-han Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Wen-han
Zhang, Hao
Guo, Qi
Wu, Xuan-di
Xu, Zi-sen
Dang, Cheng-xue
Xia, Peng
Song, Yong-chun
Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
title Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
title_full Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
title_fullStr Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
title_full_unstemmed Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
title_short Detection of SNCA and FBN1 Methylation in the Stool as a Biomarker for Colorectal Cancer
title_sort detection of snca and fbn1 methylation in the stool as a biomarker for colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353443/
https://www.ncbi.nlm.nih.gov/pubmed/25802477
http://dx.doi.org/10.1155/2015/657570
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