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Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway
The microbial source, which includes live, attenuated, or genetically modified microbes or their cellular component(s) or metabolites, has gained increasing significance for therapeutic intervention against several pathophysiological conditions of disease including leukemia, which remains an incurab...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353703/ https://www.ncbi.nlm.nih.gov/pubmed/25750993 http://dx.doi.org/10.1371/journal.pone.0120509 |
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author | Chatterjee, Nabanita Das, Subhadip Bose, Dipayan Banerjee, Somenath Jha, Tarun Das Saha, Krishna |
author_facet | Chatterjee, Nabanita Das, Subhadip Bose, Dipayan Banerjee, Somenath Jha, Tarun Das Saha, Krishna |
author_sort | Chatterjee, Nabanita |
collection | PubMed |
description | The microbial source, which includes live, attenuated, or genetically modified microbes or their cellular component(s) or metabolites, has gained increasing significance for therapeutic intervention against several pathophysiological conditions of disease including leukemia, which remains an incurable disease till now despite recent advances in the medical sciences. We therefore took up the present study to explore if the leishmanial lipid (pLLD) isolated from L. donovani can play an anti-neoplastic role in acute myeloid leukemia cells by regulating cellular growth. Indeed pLLD significantly inhibited cell proliferation of four AML cell lines (HL-60, MOLT-4, U937, and K562). Scanning electron microscopy and DNA fragmentation analysis revealed that it significantly induced apoptosis of U937 cells through morphological alteration. Occurrence of apoptosis was checked by using Annexin exposure and this established that the cell cycle was arrested at G0/G1 phase in time-dependent manner. pLLD increased the intracellular ROS with alteration of mitochondrial membrane potential, as detected using DCFDA. It also regulated the expression of apoptosis-related proteins like Bax, Bcl2, Bad and t-Bid besides causing cleavage of PARP as determined by western blot analysis. Treatment of U937 cells with pLLD induced the activation of extracellular signal-regulated kinase (ERK)1/2, c-Jun N-terminal kinase (JNK)1/2, p38, and caspases 9/3. The results suggest that pLLD induces apoptosis in acute myeloid leukemia cells possibly via increasing intracellular ROS and regulating the MAPK pathway. |
format | Online Article Text |
id | pubmed-4353703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43537032015-03-17 Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway Chatterjee, Nabanita Das, Subhadip Bose, Dipayan Banerjee, Somenath Jha, Tarun Das Saha, Krishna PLoS One Research Article The microbial source, which includes live, attenuated, or genetically modified microbes or their cellular component(s) or metabolites, has gained increasing significance for therapeutic intervention against several pathophysiological conditions of disease including leukemia, which remains an incurable disease till now despite recent advances in the medical sciences. We therefore took up the present study to explore if the leishmanial lipid (pLLD) isolated from L. donovani can play an anti-neoplastic role in acute myeloid leukemia cells by regulating cellular growth. Indeed pLLD significantly inhibited cell proliferation of four AML cell lines (HL-60, MOLT-4, U937, and K562). Scanning electron microscopy and DNA fragmentation analysis revealed that it significantly induced apoptosis of U937 cells through morphological alteration. Occurrence of apoptosis was checked by using Annexin exposure and this established that the cell cycle was arrested at G0/G1 phase in time-dependent manner. pLLD increased the intracellular ROS with alteration of mitochondrial membrane potential, as detected using DCFDA. It also regulated the expression of apoptosis-related proteins like Bax, Bcl2, Bad and t-Bid besides causing cleavage of PARP as determined by western blot analysis. Treatment of U937 cells with pLLD induced the activation of extracellular signal-regulated kinase (ERK)1/2, c-Jun N-terminal kinase (JNK)1/2, p38, and caspases 9/3. The results suggest that pLLD induces apoptosis in acute myeloid leukemia cells possibly via increasing intracellular ROS and regulating the MAPK pathway. Public Library of Science 2015-03-09 /pmc/articles/PMC4353703/ /pubmed/25750993 http://dx.doi.org/10.1371/journal.pone.0120509 Text en © 2015 Chatterjee et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chatterjee, Nabanita Das, Subhadip Bose, Dipayan Banerjee, Somenath Jha, Tarun Das Saha, Krishna Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway |
title | Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway |
title_full | Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway |
title_fullStr | Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway |
title_full_unstemmed | Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway |
title_short | Lipid from Infective L. donovani Regulates Acute Myeloid Cell Growth via Mitochondria Dependent MAPK Pathway |
title_sort | lipid from infective l. donovani regulates acute myeloid cell growth via mitochondria dependent mapk pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353703/ https://www.ncbi.nlm.nih.gov/pubmed/25750993 http://dx.doi.org/10.1371/journal.pone.0120509 |
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