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Significance of resistin expression in acute pancreatitis
The aim of the present study was to detect the expression of resistin in rats with acute pancreatitis (AP) and investigate its significance in the pathogenesis of AP. In total, 40 Sprague-Dawley rats were randomly divided into four groups (n=10), including the normal control, sham-operated, acute ed...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353746/ https://www.ncbi.nlm.nih.gov/pubmed/25780448 http://dx.doi.org/10.3892/etm.2015.2270 |
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author | XUE, LE-NING WANG, XIAO-YONG TAN, YONG LIN, MIN ZHANG, WEI XU, KE-QUN |
author_facet | XUE, LE-NING WANG, XIAO-YONG TAN, YONG LIN, MIN ZHANG, WEI XU, KE-QUN |
author_sort | XUE, LE-NING |
collection | PubMed |
description | The aim of the present study was to detect the expression of resistin in rats with acute pancreatitis (AP) and investigate its significance in the pathogenesis of AP. In total, 40 Sprague-Dawley rats were randomly divided into four groups (n=10), including the normal control, sham-operated, acute edematous pancreatitis (AEP) and acute necrotizing pancreatitis (ANP) groups. Following the establishment of animal models, the levels of serum resistin, C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β were measured using ELISA. Resistin expression in the pancreatic tissues was detected using an immunohistochemical method. In addition, the mRNA expression of resistin in the pancreatic tissues was analyzed with quantitative polymerase chain reaction. The levels of serum amylase, serum resistin, TNF-α, IL-1β and CRP were all significantly higher in the AEP and ANP groups when compared with the control and sham-operated groups (P<0.01), as were the pancreas/body weight ratios and pathological scores of the pancreas. These increases were more significant in the ANP group than in the AEP group (P<0.05). The mRNA expression levels of resistin in the pancreatic tissues were markedly higher in the AEP and ANP groups when compared with the control and sham-operated groups (P<0.01), particularly in the pancreatic tissues of the ANP group, which exhibited notably higher levels compared with the AEP group. The serum resistin level was found to positively correlate with the serum levels of CRP, TNF-α and IL-1β, and the pathological scores of the pancreatic tissues. In conclusion, the results indicated that resistin may be associated with the occurrence and development of AP; thus, the protein may be a valuable indicator for assessing the severity of AP. |
format | Online Article Text |
id | pubmed-4353746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-43537462015-03-16 Significance of resistin expression in acute pancreatitis XUE, LE-NING WANG, XIAO-YONG TAN, YONG LIN, MIN ZHANG, WEI XU, KE-QUN Exp Ther Med Articles The aim of the present study was to detect the expression of resistin in rats with acute pancreatitis (AP) and investigate its significance in the pathogenesis of AP. In total, 40 Sprague-Dawley rats were randomly divided into four groups (n=10), including the normal control, sham-operated, acute edematous pancreatitis (AEP) and acute necrotizing pancreatitis (ANP) groups. Following the establishment of animal models, the levels of serum resistin, C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β were measured using ELISA. Resistin expression in the pancreatic tissues was detected using an immunohistochemical method. In addition, the mRNA expression of resistin in the pancreatic tissues was analyzed with quantitative polymerase chain reaction. The levels of serum amylase, serum resistin, TNF-α, IL-1β and CRP were all significantly higher in the AEP and ANP groups when compared with the control and sham-operated groups (P<0.01), as were the pancreas/body weight ratios and pathological scores of the pancreas. These increases were more significant in the ANP group than in the AEP group (P<0.05). The mRNA expression levels of resistin in the pancreatic tissues were markedly higher in the AEP and ANP groups when compared with the control and sham-operated groups (P<0.01), particularly in the pancreatic tissues of the ANP group, which exhibited notably higher levels compared with the AEP group. The serum resistin level was found to positively correlate with the serum levels of CRP, TNF-α and IL-1β, and the pathological scores of the pancreatic tissues. In conclusion, the results indicated that resistin may be associated with the occurrence and development of AP; thus, the protein may be a valuable indicator for assessing the severity of AP. D.A. Spandidos 2015-04 2015-02-06 /pmc/articles/PMC4353746/ /pubmed/25780448 http://dx.doi.org/10.3892/etm.2015.2270 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles XUE, LE-NING WANG, XIAO-YONG TAN, YONG LIN, MIN ZHANG, WEI XU, KE-QUN Significance of resistin expression in acute pancreatitis |
title | Significance of resistin expression in acute pancreatitis |
title_full | Significance of resistin expression in acute pancreatitis |
title_fullStr | Significance of resistin expression in acute pancreatitis |
title_full_unstemmed | Significance of resistin expression in acute pancreatitis |
title_short | Significance of resistin expression in acute pancreatitis |
title_sort | significance of resistin expression in acute pancreatitis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353746/ https://www.ncbi.nlm.nih.gov/pubmed/25780448 http://dx.doi.org/10.3892/etm.2015.2270 |
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