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The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs

Background. The Affymetrix Drug Metabolizing Enzymes and Transporters (DMET) Plus Premier Pack has been designed to genotype 1936 gene variants thought to be essential for screening patients in personalized drug therapy. These variants include the cytochrome P450s (CYP450s), the key metabolizing enz...

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Autores principales: Wakil, Salma M., Nguyen, Cao, Muiya, Nzioka P., Andres, Editha, Lykowska-Tarnowska, Agnieszka, Baz, Batoul, Tahir, Asma I., Meyer, Brian F., Morahan, Grant, Dzimiri, Nduna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353852/
https://www.ncbi.nlm.nih.gov/pubmed/25802476
http://dx.doi.org/10.1155/2015/542543
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author Wakil, Salma M.
Nguyen, Cao
Muiya, Nzioka P.
Andres, Editha
Lykowska-Tarnowska, Agnieszka
Baz, Batoul
Tahir, Asma I.
Meyer, Brian F.
Morahan, Grant
Dzimiri, Nduna
author_facet Wakil, Salma M.
Nguyen, Cao
Muiya, Nzioka P.
Andres, Editha
Lykowska-Tarnowska, Agnieszka
Baz, Batoul
Tahir, Asma I.
Meyer, Brian F.
Morahan, Grant
Dzimiri, Nduna
author_sort Wakil, Salma M.
collection PubMed
description Background. The Affymetrix Drug Metabolizing Enzymes and Transporters (DMET) Plus Premier Pack has been designed to genotype 1936 gene variants thought to be essential for screening patients in personalized drug therapy. These variants include the cytochrome P450s (CYP450s), the key metabolizing enzymes, many other enzymes involved in phase I and phase II pharmacokinetic reactions, and signaling mediators associated with variability in clinical response to numerous drugs not only among individuals, but also between ethnic populations. Materials and Methods. We genotyped 600 Saudi individuals for 1936 variants on the DMET platform to evaluate their clinical potential in personalized medicine in ethnic Arabs. Results. Approximately 49% each of the 437 CYP450 variants, 56% of the 581 transporters, 56% of 419 transferases, 48% of the 104 dehydrogenases, and 58% of the remaining 390 variants were detected. Several variants, such as rs3740071, rs6193, rs258751, rs6199, rs11568421, and rs8187797, exhibited significantly either higher or lower minor allele frequencies (MAFs) than those in other ethnic groups. Discussion. The present study revealed some unique distribution trends for several variants in Arabs, which displayed partly inverse allelic prevalence compared to other ethnic populations. The results point therefore to the need to verify and ascertain the prevalence of a variant as a prerequisite for engaging it in clinical routine screening in personalized medicine in any given population.
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spelling pubmed-43538522015-03-23 The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs Wakil, Salma M. Nguyen, Cao Muiya, Nzioka P. Andres, Editha Lykowska-Tarnowska, Agnieszka Baz, Batoul Tahir, Asma I. Meyer, Brian F. Morahan, Grant Dzimiri, Nduna Dis Markers Research Article Background. The Affymetrix Drug Metabolizing Enzymes and Transporters (DMET) Plus Premier Pack has been designed to genotype 1936 gene variants thought to be essential for screening patients in personalized drug therapy. These variants include the cytochrome P450s (CYP450s), the key metabolizing enzymes, many other enzymes involved in phase I and phase II pharmacokinetic reactions, and signaling mediators associated with variability in clinical response to numerous drugs not only among individuals, but also between ethnic populations. Materials and Methods. We genotyped 600 Saudi individuals for 1936 variants on the DMET platform to evaluate their clinical potential in personalized medicine in ethnic Arabs. Results. Approximately 49% each of the 437 CYP450 variants, 56% of the 581 transporters, 56% of 419 transferases, 48% of the 104 dehydrogenases, and 58% of the remaining 390 variants were detected. Several variants, such as rs3740071, rs6193, rs258751, rs6199, rs11568421, and rs8187797, exhibited significantly either higher or lower minor allele frequencies (MAFs) than those in other ethnic groups. Discussion. The present study revealed some unique distribution trends for several variants in Arabs, which displayed partly inverse allelic prevalence compared to other ethnic populations. The results point therefore to the need to verify and ascertain the prevalence of a variant as a prerequisite for engaging it in clinical routine screening in personalized medicine in any given population. Hindawi Publishing Corporation 2015 2015-02-22 /pmc/articles/PMC4353852/ /pubmed/25802476 http://dx.doi.org/10.1155/2015/542543 Text en Copyright © 2015 Salma M. Wakil et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wakil, Salma M.
Nguyen, Cao
Muiya, Nzioka P.
Andres, Editha
Lykowska-Tarnowska, Agnieszka
Baz, Batoul
Tahir, Asma I.
Meyer, Brian F.
Morahan, Grant
Dzimiri, Nduna
The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs
title The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs
title_full The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs
title_fullStr The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs
title_full_unstemmed The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs
title_short The Affymetrix DMET Plus Platform Reveals Unique Distribution of ADME-Related Variants in Ethnic Arabs
title_sort affymetrix dmet plus platform reveals unique distribution of adme-related variants in ethnic arabs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353852/
https://www.ncbi.nlm.nih.gov/pubmed/25802476
http://dx.doi.org/10.1155/2015/542543
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