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Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops

Samples of two Ageratum conyzoides, one Sonchus oleraceus and one turnip (Brassica rapa var. rapa) exhibiting virus-like symptoms were collected from Pakistan and Nepal. Full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. The begomov...

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Autores principales: Tahir, Muhammad, Amin, Imran, Haider, Muhammad Saleem, Mansoor, Shahid, Briddon, Rob W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353908/
https://www.ncbi.nlm.nih.gov/pubmed/25674770
http://dx.doi.org/10.3390/v7020647
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author Tahir, Muhammad
Amin, Imran
Haider, Muhammad Saleem
Mansoor, Shahid
Briddon, Rob W.
author_facet Tahir, Muhammad
Amin, Imran
Haider, Muhammad Saleem
Mansoor, Shahid
Briddon, Rob W.
author_sort Tahir, Muhammad
collection PubMed
description Samples of two Ageratum conyzoides, one Sonchus oleraceus and one turnip (Brassica rapa var. rapa) exhibiting virus-like symptoms were collected from Pakistan and Nepal. Full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. The begomovirus sequences were shown to be isolates of Ageratum enation virus (AEV) with greater than 89.1% nucleotide sequence identity to the 26 AEV sequences available in the databases. The three betasatellite sequences were shown to be isolates of Ageratum yellow leaf curl betasatellite (AYLCB) with greater than 90% identity to the 18 AYLCB sequences available in the databases. The AEV sequences were shown to fall into two distinct strains, for which the names Nepal (consisting of isolates from Nepal, India, and Pakistan—including the isolates identified here) and India (isolates occurring only in India) strains are proposed. For the clones obtained from two AEV isolates, with their AYLCB, infectivity was shown by Agrobacterium-mediated inoculation to Nicotiana benthamiana, N. tabacum, Solanum lycopersicon and A. conyzoides. N. benthamiana plants infected with AEV alone or betasatellite alone showed no symptoms. N. benthamiana plants infected with AEV with its associated betasatellite showed leaf curl symptoms. The findings show that AEV is predominantly a virus of weeds that has the capacity to infect crops. AYLCB appears to be the common partner betasatellite of AEV and is associated with diseases with a range of very different symptoms in the same plant species. The inability to satisfy Koch’s postulates with the cloned components of isolate SOL in A. conyzoides suggests that the etiology may be more complex than a single virus with a single betasatellite.
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spelling pubmed-43539082015-04-10 Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops Tahir, Muhammad Amin, Imran Haider, Muhammad Saleem Mansoor, Shahid Briddon, Rob W. Viruses Article Samples of two Ageratum conyzoides, one Sonchus oleraceus and one turnip (Brassica rapa var. rapa) exhibiting virus-like symptoms were collected from Pakistan and Nepal. Full-length begomovirus clones were obtained from the four plant samples and betasatellite clones from three of these. The begomovirus sequences were shown to be isolates of Ageratum enation virus (AEV) with greater than 89.1% nucleotide sequence identity to the 26 AEV sequences available in the databases. The three betasatellite sequences were shown to be isolates of Ageratum yellow leaf curl betasatellite (AYLCB) with greater than 90% identity to the 18 AYLCB sequences available in the databases. The AEV sequences were shown to fall into two distinct strains, for which the names Nepal (consisting of isolates from Nepal, India, and Pakistan—including the isolates identified here) and India (isolates occurring only in India) strains are proposed. For the clones obtained from two AEV isolates, with their AYLCB, infectivity was shown by Agrobacterium-mediated inoculation to Nicotiana benthamiana, N. tabacum, Solanum lycopersicon and A. conyzoides. N. benthamiana plants infected with AEV alone or betasatellite alone showed no symptoms. N. benthamiana plants infected with AEV with its associated betasatellite showed leaf curl symptoms. The findings show that AEV is predominantly a virus of weeds that has the capacity to infect crops. AYLCB appears to be the common partner betasatellite of AEV and is associated with diseases with a range of very different symptoms in the same plant species. The inability to satisfy Koch’s postulates with the cloned components of isolate SOL in A. conyzoides suggests that the etiology may be more complex than a single virus with a single betasatellite. MDPI 2015-02-10 /pmc/articles/PMC4353908/ /pubmed/25674770 http://dx.doi.org/10.3390/v7020647 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tahir, Muhammad
Amin, Imran
Haider, Muhammad Saleem
Mansoor, Shahid
Briddon, Rob W.
Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops
title Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops
title_full Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops
title_fullStr Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops
title_full_unstemmed Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops
title_short Ageratum enation virus—A Begomovirus of Weeds with the Potential to Infect Crops
title_sort ageratum enation virus—a begomovirus of weeds with the potential to infect crops
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353908/
https://www.ncbi.nlm.nih.gov/pubmed/25674770
http://dx.doi.org/10.3390/v7020647
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