Cargando…

Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture

Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Min Kyung, Rich, Max H., Baek, Kwanghyun, Lee, Jonghwi, Kong, Hyunjoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353999/
https://www.ncbi.nlm.nih.gov/pubmed/25752700
http://dx.doi.org/10.1038/srep08948
_version_ 1782360686174666752
author Lee, Min Kyung
Rich, Max H.
Baek, Kwanghyun
Lee, Jonghwi
Kong, Hyunjoon
author_facet Lee, Min Kyung
Rich, Max H.
Baek, Kwanghyun
Lee, Jonghwi
Kong, Hyunjoon
author_sort Lee, Min Kyung
collection PubMed
description Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable gel, because the gel becomes less permeable with increasing rigidity. To resolve this challenge, this study demonstrates a unique method to enhance the permeability of a cell-laden hydrogel while avoiding a significant change in rigidity of the gel. Inspired by the grooved skin textures of marine organisms, a hydrogel is assembled to present computationally optimized micro-sized grooves on the surface. Separately, a gel is engineered to preset aligned microchannels similar to a plant's vascular bundles through a uniaxial freeze-drying process. The resulting gel displays significantly increased water diffusivity with reduced changes of gel stiffness, exclusively when the microgrooves and microchannels are aligned together. No significant enhancement of rehydration is achieved when the microgrooves and microchannels are not aligned. Such material design greatly enhances viability and neural differentiation of stem cells and 3D neural network formation within the gel.
format Online
Article
Text
id pubmed-4353999
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-43539992015-03-17 Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture Lee, Min Kyung Rich, Max H. Baek, Kwanghyun Lee, Jonghwi Kong, Hyunjoon Sci Rep Article Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable gel, because the gel becomes less permeable with increasing rigidity. To resolve this challenge, this study demonstrates a unique method to enhance the permeability of a cell-laden hydrogel while avoiding a significant change in rigidity of the gel. Inspired by the grooved skin textures of marine organisms, a hydrogel is assembled to present computationally optimized micro-sized grooves on the surface. Separately, a gel is engineered to preset aligned microchannels similar to a plant's vascular bundles through a uniaxial freeze-drying process. The resulting gel displays significantly increased water diffusivity with reduced changes of gel stiffness, exclusively when the microgrooves and microchannels are aligned together. No significant enhancement of rehydration is achieved when the microgrooves and microchannels are not aligned. Such material design greatly enhances viability and neural differentiation of stem cells and 3D neural network formation within the gel. Nature Publishing Group 2015-03-10 /pmc/articles/PMC4353999/ /pubmed/25752700 http://dx.doi.org/10.1038/srep08948 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lee, Min Kyung
Rich, Max H.
Baek, Kwanghyun
Lee, Jonghwi
Kong, Hyunjoon
Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture
title Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture
title_full Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture
title_fullStr Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture
title_full_unstemmed Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture
title_short Bioinspired Tuning of Hydrogel Permeability-Rigidity Dependency for 3D Cell Culture
title_sort bioinspired tuning of hydrogel permeability-rigidity dependency for 3d cell culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353999/
https://www.ncbi.nlm.nih.gov/pubmed/25752700
http://dx.doi.org/10.1038/srep08948
work_keys_str_mv AT leeminkyung bioinspiredtuningofhydrogelpermeabilityrigiditydependencyfor3dcellculture
AT richmaxh bioinspiredtuningofhydrogelpermeabilityrigiditydependencyfor3dcellculture
AT baekkwanghyun bioinspiredtuningofhydrogelpermeabilityrigiditydependencyfor3dcellculture
AT leejonghwi bioinspiredtuningofhydrogelpermeabilityrigiditydependencyfor3dcellculture
AT konghyunjoon bioinspiredtuningofhydrogelpermeabilityrigiditydependencyfor3dcellculture