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Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador
The Province of Santo Domingo de los Tsáchilas in Ecuador represents the largest informal cattle market. Because of its strategic position, cattle movement is very high and therefore we selected this region, to determine the strain variation of Brucella sp. Part of the study aimed at the isolation,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354208/ https://www.ncbi.nlm.nih.gov/pubmed/25806363 http://dx.doi.org/10.3389/fpubh.2015.00045 |
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author | Rodríguez-Hidalgo, Richar Ivan Contreras-Zamora, Javier Benitez Ortiz, Washington Guerrero-Viracocha, Karina Salcan-Guaman, Holger Minda, Elizabeth Ron Garrido, Lenin |
author_facet | Rodríguez-Hidalgo, Richar Ivan Contreras-Zamora, Javier Benitez Ortiz, Washington Guerrero-Viracocha, Karina Salcan-Guaman, Holger Minda, Elizabeth Ron Garrido, Lenin |
author_sort | Rodríguez-Hidalgo, Richar Ivan |
collection | PubMed |
description | The Province of Santo Domingo de los Tsáchilas in Ecuador represents the largest informal cattle market. Because of its strategic position, cattle movement is very high and therefore we selected this region, to determine the strain variation of Brucella sp. Part of the study aimed at the isolation, biotyping, and genotyping of Brucella species from milk and supra-mammary lymph nodes of sero-positive bovines, using selective Farrell medium, biochemical assays, and IS711-PCR, AMOS-PCR, and HOOF-Prints techniques. In total, 656 animals from 12 sero-positive dairy herds and from the provincial slaughterhouse were diagnosed by Rose Bengal and Wright’s Slow Agglutination test with EDTA. Amongst these animals, 50 animals were sero-positive for brucellosis. Twenty-five lymph nodes and 25 milk samples from each group of positive reactors were transferred to culture medium. Isolation was possible from 4 (16%) lymph nodes and 9 (36%) milk samples; out of these, 10 isolates were diagnosed as Brucella sp. All four isolates of lymphatic tissue corresponded to Brucella abortus biotype 1, confirmed as field strains by molecular analysis. Milk isolations, showed biochemically a more dispersed pattern in which B. abortus biotypes 1 and 4 were found; yet four samples gave a pattern similar to B. abortus biotype 2; however, only biotypes 1 and 4 were confirmed by molecular analysis. The concordance between biochemical and molecular diagnostic tests reached 76.9%. |
format | Online Article Text |
id | pubmed-4354208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43542082015-03-24 Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador Rodríguez-Hidalgo, Richar Ivan Contreras-Zamora, Javier Benitez Ortiz, Washington Guerrero-Viracocha, Karina Salcan-Guaman, Holger Minda, Elizabeth Ron Garrido, Lenin Front Public Health Public Health The Province of Santo Domingo de los Tsáchilas in Ecuador represents the largest informal cattle market. Because of its strategic position, cattle movement is very high and therefore we selected this region, to determine the strain variation of Brucella sp. Part of the study aimed at the isolation, biotyping, and genotyping of Brucella species from milk and supra-mammary lymph nodes of sero-positive bovines, using selective Farrell medium, biochemical assays, and IS711-PCR, AMOS-PCR, and HOOF-Prints techniques. In total, 656 animals from 12 sero-positive dairy herds and from the provincial slaughterhouse were diagnosed by Rose Bengal and Wright’s Slow Agglutination test with EDTA. Amongst these animals, 50 animals were sero-positive for brucellosis. Twenty-five lymph nodes and 25 milk samples from each group of positive reactors were transferred to culture medium. Isolation was possible from 4 (16%) lymph nodes and 9 (36%) milk samples; out of these, 10 isolates were diagnosed as Brucella sp. All four isolates of lymphatic tissue corresponded to Brucella abortus biotype 1, confirmed as field strains by molecular analysis. Milk isolations, showed biochemically a more dispersed pattern in which B. abortus biotypes 1 and 4 were found; yet four samples gave a pattern similar to B. abortus biotype 2; however, only biotypes 1 and 4 were confirmed by molecular analysis. The concordance between biochemical and molecular diagnostic tests reached 76.9%. Frontiers Media S.A. 2015-03-10 /pmc/articles/PMC4354208/ /pubmed/25806363 http://dx.doi.org/10.3389/fpubh.2015.00045 Text en Copyright © 2015 Rodríguez-Hidalgo, Contreras-Zamora, Benitez Ortiz, Guerrero-Viracocha, Salcan-Guaman, Minda and Ron Garrido. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Public Health Rodríguez-Hidalgo, Richar Ivan Contreras-Zamora, Javier Benitez Ortiz, Washington Guerrero-Viracocha, Karina Salcan-Guaman, Holger Minda, Elizabeth Ron Garrido, Lenin Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador |
title | Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador |
title_full | Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador |
title_fullStr | Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador |
title_full_unstemmed | Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador |
title_short | Circulating Strains of Brucella abortus in Cattle in Santo Domingo De Los Tsáchilas Province – Ecuador |
title_sort | circulating strains of brucella abortus in cattle in santo domingo de los tsáchilas province – ecuador |
topic | Public Health |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354208/ https://www.ncbi.nlm.nih.gov/pubmed/25806363 http://dx.doi.org/10.3389/fpubh.2015.00045 |
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