Cargando…
Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis
Insulin-like growth factor plays a paracrine/autocrine role in regulating testicular function in the stallion, but its presence in the equine epididymis remains unknown. The aim of this study was to test the hypothesis that insulin-like growth factor-I (IGF-I) and IGF-I receptor (IGF-IR) are localiz...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354228/ https://www.ncbi.nlm.nih.gov/pubmed/25311540 http://dx.doi.org/10.1262/jrd.2014-097 |
_version_ | 1782360724635385856 |
---|---|
author | YOON, Minjung JIANG, Juliann CHUNG, Ki Hwa ROSER, Janet Fay |
author_facet | YOON, Minjung JIANG, Juliann CHUNG, Ki Hwa ROSER, Janet Fay |
author_sort | YOON, Minjung |
collection | PubMed |
description | Insulin-like growth factor plays a paracrine/autocrine role in regulating testicular function in the stallion, but its presence in the equine epididymis remains unknown. The aim of this study was to test the hypothesis that insulin-like growth factor-I (IGF-I) and IGF-I receptor (IGF-IR) are localized in the caput, corpus, and cauda of the epididymis in an age-dependent manner. Immediately after castration, epididymal tissue was fixed, paraffin-embedded, and processed for immunohistochemistry (IHC). Western blot was also performed using equine epididymal extracts to verify the specificity of the antibodies against IGF-I and IGF-IR. Immunolabeling of IGF-I was observed in the cytoplasm of principal and basal cells in the caput, corpus, and cauda at the pre-pubertal (3–7 months), pubertal (12–18 months), post-pubertal (2–4 years), and adult stages (4.5–8 years). Immunolabeling of IGF-IR was observed in the cytoplasm of principal cells in all regions of the epididymis in each age group. Immunolabeling of IGF-IR was also detected in the cytoplasm of basal cells from animals of all ages. Bands observed by Western blot corresponded to the molecular weights of IGF-I and IGF-IR, ~23 kDa and 95 kDa, respectively. These results suggest that IGF-I might function as an autocrine and/or paracrine factor during the development, maintenance and/or secretions of the stallion epididymis. |
format | Online Article Text |
id | pubmed-4354228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-43542282015-03-13 Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis YOON, Minjung JIANG, Juliann CHUNG, Ki Hwa ROSER, Janet Fay J Reprod Dev Original Article Insulin-like growth factor plays a paracrine/autocrine role in regulating testicular function in the stallion, but its presence in the equine epididymis remains unknown. The aim of this study was to test the hypothesis that insulin-like growth factor-I (IGF-I) and IGF-I receptor (IGF-IR) are localized in the caput, corpus, and cauda of the epididymis in an age-dependent manner. Immediately after castration, epididymal tissue was fixed, paraffin-embedded, and processed for immunohistochemistry (IHC). Western blot was also performed using equine epididymal extracts to verify the specificity of the antibodies against IGF-I and IGF-IR. Immunolabeling of IGF-I was observed in the cytoplasm of principal and basal cells in the caput, corpus, and cauda at the pre-pubertal (3–7 months), pubertal (12–18 months), post-pubertal (2–4 years), and adult stages (4.5–8 years). Immunolabeling of IGF-IR was observed in the cytoplasm of principal cells in all regions of the epididymis in each age group. Immunolabeling of IGF-IR was also detected in the cytoplasm of basal cells from animals of all ages. Bands observed by Western blot corresponded to the molecular weights of IGF-I and IGF-IR, ~23 kDa and 95 kDa, respectively. These results suggest that IGF-I might function as an autocrine and/or paracrine factor during the development, maintenance and/or secretions of the stallion epididymis. The Society for Reproduction and Development 2014-10-12 2015-02 /pmc/articles/PMC4354228/ /pubmed/25311540 http://dx.doi.org/10.1262/jrd.2014-097 Text en ©2015 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Original Article YOON, Minjung JIANG, Juliann CHUNG, Ki Hwa ROSER, Janet Fay Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis |
title | Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis |
title_full | Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis |
title_fullStr | Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis |
title_full_unstemmed | Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis |
title_short | Immunolocalization of insulin-like growth factor-I (IGF-I) and its receptors (IGF-IR) in the equine epididymis |
title_sort | immunolocalization of insulin-like growth factor-i (igf-i) and its receptors (igf-ir) in the equine epididymis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354228/ https://www.ncbi.nlm.nih.gov/pubmed/25311540 http://dx.doi.org/10.1262/jrd.2014-097 |
work_keys_str_mv | AT yoonminjung immunolocalizationofinsulinlikegrowthfactoriigfianditsreceptorsigfirintheequineepididymis AT jiangjuliann immunolocalizationofinsulinlikegrowthfactoriigfianditsreceptorsigfirintheequineepididymis AT chungkihwa immunolocalizationofinsulinlikegrowthfactoriigfianditsreceptorsigfirintheequineepididymis AT roserjanetfay immunolocalizationofinsulinlikegrowthfactoriigfianditsreceptorsigfirintheequineepididymis |