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Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway

Breast cancer is considered to be the most common malignancy in women. Treatment of breast cancer has been focused on molecular targeted therapy, and anticancer peptides are considered to be some of the most promising candidate drugs. In the current study, we used mRNA-peptide display technology to...

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Autores principales: Yang, Longfei, Cui, Ying, Shen, Jianjun, Lin, Fang, Wang, Xi, Long, Min, Wei, Junxia, Zhang, Huizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354433/
https://www.ncbi.nlm.nih.gov/pubmed/25767377
http://dx.doi.org/10.2147/DDDT.S75780
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author Yang, Longfei
Cui, Ying
Shen, Jianjun
Lin, Fang
Wang, Xi
Long, Min
Wei, Junxia
Zhang, Huizhong
author_facet Yang, Longfei
Cui, Ying
Shen, Jianjun
Lin, Fang
Wang, Xi
Long, Min
Wei, Junxia
Zhang, Huizhong
author_sort Yang, Longfei
collection PubMed
description Breast cancer is considered to be the most common malignancy in women. Treatment of breast cancer has been focused on molecular targeted therapy, and anticancer peptides are considered to be some of the most promising candidate drugs. In the current study, we used mRNA-peptide display technology to screen antibreast cancer peptides and identified a novel peptide, SA12, which showed significant activity in the inhibition of proliferation and induction of apoptosis in SKBr-3 breast cancer cells. The mechanism by which SA12 peptide triggers apoptosis was further investigated using a pull-down assay, reverse transcription-polymerase chain reaction, and Western blotting analysis. The results demonstrated that this peptide could interact with tumor-associated proteins MECP2 and CDC20B, which further induced apoptosis of tumor cells via the mitochondrial pathway involving the Bcl-2 family and related caspases. We propose that the novel SA12 peptide has the potential to provide a new strategy for the development of targeted therapy in breast cancer.
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spelling pubmed-43544332015-03-12 Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway Yang, Longfei Cui, Ying Shen, Jianjun Lin, Fang Wang, Xi Long, Min Wei, Junxia Zhang, Huizhong Drug Des Devel Ther Original Research Breast cancer is considered to be the most common malignancy in women. Treatment of breast cancer has been focused on molecular targeted therapy, and anticancer peptides are considered to be some of the most promising candidate drugs. In the current study, we used mRNA-peptide display technology to screen antibreast cancer peptides and identified a novel peptide, SA12, which showed significant activity in the inhibition of proliferation and induction of apoptosis in SKBr-3 breast cancer cells. The mechanism by which SA12 peptide triggers apoptosis was further investigated using a pull-down assay, reverse transcription-polymerase chain reaction, and Western blotting analysis. The results demonstrated that this peptide could interact with tumor-associated proteins MECP2 and CDC20B, which further induced apoptosis of tumor cells via the mitochondrial pathway involving the Bcl-2 family and related caspases. We propose that the novel SA12 peptide has the potential to provide a new strategy for the development of targeted therapy in breast cancer. Dove Medical Press 2015-03-02 /pmc/articles/PMC4354433/ /pubmed/25767377 http://dx.doi.org/10.2147/DDDT.S75780 Text en © 2015 Yang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yang, Longfei
Cui, Ying
Shen, Jianjun
Lin, Fang
Wang, Xi
Long, Min
Wei, Junxia
Zhang, Huizhong
Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway
title Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway
title_full Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway
title_fullStr Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway
title_full_unstemmed Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway
title_short Antitumor activity of SA12, a novel peptide, on SKBr-3 breast cancer cells via the mitochondrial apoptosis pathway
title_sort antitumor activity of sa12, a novel peptide, on skbr-3 breast cancer cells via the mitochondrial apoptosis pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354433/
https://www.ncbi.nlm.nih.gov/pubmed/25767377
http://dx.doi.org/10.2147/DDDT.S75780
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