Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Gene targeting refers to the precise modification of a genetic locus using homologous recombination. The generation of novel cell lines and transgenic mouse models using this method necessitates the construction of a ‘targeting’ vector, which contains homologous DNA sequences to the target gene, and...

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Detalles Bibliográficos
Autores principales: Reddy, Thimma R., Kelsall, Emma J., Fevat, Léna M.S., Munson, Sarah E., Cowley, Shaun M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354499/
https://www.ncbi.nlm.nih.gov/pubmed/25590226
http://dx.doi.org/10.3791/52155

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354499/
https://www.ncbi.nlm.nih.gov/pubmed/25590226
http://dx.doi.org/10.3791/52155

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