Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

The production of recombinant membrane proteins for structural and functional studies remains technically challenging due to low levels of expression and the inherent instability of many membrane proteins once solubilized in detergents. A protocol is described that combines ligation independent clon...

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Detalles Bibliográficos
Autores principales: Bird, Louise E., Rada, Heather, Verma, Anil, Gasper, Raphael, Birch, James, Jennions, Matthew, Lӧwe, Jan, Moraes, Isabel, Owens, Raymond J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2015
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354503/
https://www.ncbi.nlm.nih.gov/pubmed/25590335
http://dx.doi.org/10.3791/52357

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4354503/
https://www.ncbi.nlm.nih.gov/pubmed/25590335
http://dx.doi.org/10.3791/52357

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